HPLC梯度法测定复方三七维康胶囊中三七皂苷R_1、人参皂苷R_(b1)、R_(g1)的含量  

Determination of Notoginsenoside R_1 and Ginsenoside R_(g1) and R_(b1) in Compound Sanqi Weikang Capsules by HPLC

作　　者：孙小玲[1] 

机构地区：[1]辽宁省食品药品检验所,沈阳110023

出　　处：《中国药师》2013年第3期349-351,共3页China Pharmacist

摘　　要：目的:建立HPLC梯度法同时测定复方三七维康胶囊中三七皂苷R_1、人参皂苷R_(b1)、R_(g1)3种皂苷的含量。方法:色谱柱:大连Spherisorb C_(18)分析柱(200 mm×4.6 mm,5μm);流动相:乙腈-水,梯度洗脱;流速:1.0 ml·min^(-1);检测波长203 nm。结果:三七皂苷R_1、人参皂苷R_(gl)和R_(b1)分别在1.06～18.55μg(r=0.997 3)、2.02～35.35μg(r=0.998 2)和2.02～35.35μg(r=0.998 2)之间线性关系良好。平均回收率三七皂苷R_1为100.8%(RSD=1.53%,n=5),人参皂苷R_1为98.9%(RSD=1.87%,n=5),人参皂苷R_(b1)为99.7%(RSD=1.90%,n=5)。结论:HPLC梯度洗脱法能够将多种皂苷很好地分离检测,该方法准确可靠,重复性好,可用于控制其质量。Objective：To establish an HPLC method for the determination of notoginsenoside R_1,ginsenoside R_（g1） and R_（b1）in compound Sanqi Weikang capsules.Method：A Spherisorb C_（18）（200 mm×4.6 mm,5μm） column was used with the mobile phase of acetonitrile-water with gradient elution.The flow rate was 1.0 ml·min^（-1）,and the detection wavelength was set at 203 nm.Result： The linear range of notoginsenoside R_1 was 1.06-18.55μg（r = 0.997 3）,2.02-35.35μg（r = 0.998 2） and 2.02-35.35μg（r = 0.998 2）,respectively,the average recovery was 100.8%（RSD = 1.53%,n = 5）,98.9%（RSD = 1.87%,n = 5） and 99.7%（RSD = 1.90%,n=5）,respectively.Conclusion：The method is rapid,accurate,sensitive and reliable,and can be used to control the quality of the capsules.

关 键 词：高效液相色谱法 三七皂苷R_1 人参皂苷R_(b1) 人参皂苷R_(g1) 复方三七维康胶囊 

分 类 号：R927.2[医药卫生—药学]