磷酸肌酸钠对缺氧窒息幼鼠肾组织HIF-1α和VEGF表达的影响  

Effects of creatine phosphate sodium on expressions of HIF-1α and VEGF in renal tissue of anoxia asphyxia young rats

作　　者：刘月梅[1] 陈莹[1] 李永凤[1] 刘春艳[1] 

机构地区：[1]郑州大学第二附属医院儿科,郑州450014

出　　处：《郑州大学学报（医学版）》2013年第1期92-95,共4页Journal of Zhengzhou University(Medical Sciences)

摘　　要：目的:研究磷酸肌酸钠(PCr)对缺氧窒息幼鼠肾组织缺氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)表达的影响。方法:24只幼鼠随机分为PCr干预组(PCr组)、模型组和正常对照组,每组8只。PCr和模型组幼鼠制作缺氧窒息模型,PCr组幼鼠在制作模型前0.5h和缺氧窒息后即刻腹腔注射1.4mg/g的PCr。模型组在相同时间点腹腔注射等量的生理盐水。正常对照组不作处理。缺氧窒息后6h,切除各组幼鼠肾脏,采用HE染色法观察肾组织病理形态变化,RT-PCR和免疫组织化学法检测肾组织HIF-1α和VEGFmRNA和蛋白的表达。结果:模型组幼鼠肾组织可见肾小管上皮细胞肿胀,大量炎性细胞浸润,部分上皮细胞变性坏死,肾小球出现水肿、甚至固缩;PCr组幼鼠肾小管和肾小球受损状况明显减轻。3组幼鼠肾组织HIF-1α和VEGFmRNA及蛋白的表达比较,差异有统计学意义(F=196.805、484.734、90.410和79.329,P均<0.001),PCr和模型组均较正常对照组升高,PCr组较模型组降低(P<0.05)。结论:PCr可能通过下调HIF-1α、VEGF的表达减轻肾组织的缺氧性损伤。Aim：To research the influence of creatine phosphate sodium（PCr） on expressions of hypoxia-inducible factor-1 alpha（HIF-1α）and vascular endothelial growth factor（VEGF）in renal tissue of anoxia asphyxia young rats.Methods：A total of 24 young rats were randomly divided into three groups,PCr intervention group（PCr group）,model group and normal control group,8 rats in each group.After anoxia asphyxia 6 h,shape and structure of the kidney were observed using HE staining,the expressions of HIF-1α and VEGF mRNA were detected using RT-PCR and the expressions of HIF-1α and VEGF protein were detected by immunohistochemical staining.Results：Compared with normal control group,pathological changes of renal lesions were observed in renal tissue of both PCr and model groups.The expressions of VEGF and HIF-1α mRNA and protein in renal tissue among the 3 groups had significant differences（F=196.805,484.734,90.410,and 79.329,P0.001）,which were higher in both PCr and model groups than those in normal control group,and lower in PCr group than those in model group（P0.05）.Conclusion：PCr could reduce kidney damage after anoxia asphyxia by down-regulating the expressions of HIT-1α and VEGF.

关 键 词：缺氧窒息 缺氧诱导因子-1Α 血管内皮生长因子 磷酸肌酸钠 大鼠 

分 类 号：R725.7[医药卫生—儿科]