液相色谱法测定2-氨基-4-硝基苯酚  

Determination of 2-Amino-4-nitrophenol by HPLC

作　　者：季浩[1] 杨瑞莹 蒲爱军[1] 沈日炯[1] 

机构地区：[1]沈阳化工研究院有限公司,沈阳110021 [2]天津裕华经贸公司,天津300382

出　　处：《染料与染色》2013年第1期60-62,共3页Dyestuffs and Coloration

摘　　要：采用高效液相色谱对2-氨基-4-硝基苯酚及其有机杂质进行了分离,用峰面积归一化法进行定量分析。通过优化流动相的组成确定了样品分离条件,综合考虑待测物的最大紫外吸收确定了检测波长。优化的测定条件为流动相甲醇∶水=60∶40,在波长254 nm处进行测定。2-Amino-4-nitrophenol and its impurites were separated by HPLC, and then quantified with peak area normalization meth- od. The separation condition of samples was confirmed by optimizing the components of mobile phase ; the detection wavelengh was de- termined by eomprehensively considering maximum absorption wavelength of determinands. The optimized condition was mobile phase methanol： water = 60：40 and detection wavelength 254 nm.

关 键 词：2-氨基-4-硝基苯酚 高效液相色谱 峰面积归一化法 

分 类 号：TQ610.7[化学工程—精细化工]