更年期妇女与健康育龄妇女阴道菌群的比较研究  

Comparative Study of Vaginal Flora between Menopausal Women and Child-Bearing Women

作　　者：彭国丽[1] 吕治[1] 苏建荣[1] 

机构地区：[1]首都医科大学附属北京友谊医院检验中心,北京100050

出　　处：《现代检验医学杂志》2013年第1期70-73,共4页Journal of Modern Laboratory Medicine

基　　金：国家自然科学基金（30972819）.

摘　　要：目的 比较更年期妇女与健康育龄妇女阴道菌群构成及数量的差异.方法 采集10例健康育龄妇女,6例更年期妇女阴道分泌物标本,构建阴道细菌16SrDNA基因文库,对3 200个阳性克隆子进行测序鉴定.比较更年期和健康育龄期妇女阴道菌群的构成差异.采用荧光定量PCR方法比较两组标本中优势乳酸杆菌数量的差异.结果 健康妇女阴道菌群构成单一,多为2～3种细菌,乳酸杆菌属细菌占绝对优势,在更年期妇女和健康育龄期妇女分别为86.7%和99.2%.健康育龄期妇女阴道菌群构成以卷曲乳酸杆菌为主占72.2%,更年期妇女阴道菌群构成以惰性乳酸杆菌为主占81.4%.更年期妇女阴道乳酸杆菌的16SrDNA基因拷贝数对数值为5.27±0.31（Log10 copies/ml）,较健康育龄期妇女5.76±0.35（Log10 copies/ml）明显减少（z=-2.242,P〈0.05）,其中卷曲乳酸杆菌数量由育龄期的5.62±0.32（Log10 copies/ml）下降为更年期的3.74±0.42（Log10 copies/ml）,差异具有统计学意义（z=-3.256,P〈0.05）.两组标本中的惰性乳酸杆菌16SrDNA基因拷贝数差异无统计学意义（z=-0.543,P〉0.05）.结论 更年期妇女和健康育龄期妇女阴道菌群构成上存在差异,乳酸杆菌的种类和数量发生了明显改变.Objective To confirm the vaginal flora characteristics of healthy menopausal women and compare the vaginal flora differences between menopausal age and child-bearing age. Methods Broad-range PCR amplification of 16SrDNA and construction of vaginal community library were used to analyze the vaginal flora diversity of 10 child-bearing women, 6 menopause women. Total 3 200 positive clones were sequenced and identified. Fluorescence quantitative PCR were used to compare the Quantity discrepancy in two groups. Results 2 to 3 vaginal bacterial species were found in healthy women vaginal. Vaginal lactobacilli were the predominant bacterium in healthy women vaginal. Lactobacillus in menopausal women and in child-bearing women was 86.7 % and 99.2 %, respectively, Lactobacillus erispatus was the predominant bacterium in childbearing women （72.2%）. Lactobacillus iners was the predominant bacterium in menopausal women （81.4%）. Compared with child-bearing women the number of vaginal lactobacillus 16SrDNA genes of menopausal women were decreased significantly,from 5.76±0. 35 （Logl0copies/ml） to 5.27±0.31 （Log10 copies/ml）. The difference was significantly （z= - 0. 242,P〈0.05）. The number of L. crispatus 16SrDNA genes decreased from 5.62± 0.32 （Log10copies/ml） to 3.74±0. 42 （Log10copies/ml） （z=- 3. 256, P〈0.05）, No statistically significant differences in amount of L. Iners 16SrDNA genes were found among the two groups （z= -0. 543 ,P〉0.05）. Conclusion The findings indicate further diversity of bac- terial species in the women vagina in different physiology period. The species and quantity of laetobacillus in the women vagi- nal changed obviously.

关 键 词：更年期 健康育龄妇女 阴道菌群 

分 类 号：R446.5[医药卫生—诊断学]