氯丙烯对神经细胞内钙磷含量和能量合成的影响  

Effects of allyl chloride on calcium,phosphorus contents and energy synthesis in nerve cells

作　　者：谢克勤[1] 赵丽[2] 张 厉保秋[2] 孙克任[2] 阮迪云[1] 

机构地区：[1]中国科技大学生命科学学院神经毒理学实验室,安徽合肥230027 [2]山东医科大学毒理学省级重点实验室,山东济南250012

出　　处：《中国药理学与毒理学杂志》2000年第5期369-372,共4页Chinese Journal of Pharmacology and Toxicology

基　　金：山东省自然科学基金资助项目!(Y97C130 49)

摘　　要：用鸡胚脑神经细胞研究氯丙烯作用下细胞内钙 ,磷 ,ATP,ADP,AMP含量及线粒体琥珀酸脱氢酶 (SDH)活性的改变 .结果表明 ,随着氯丙烯浓度的增加 ,细胞内钙含量明显增加 ,而磷含量则明显减少 ;线粒体 SDH活性明显减低 ,并有明显的浓度 -效应关系 (r=- 0 .9957) ;细胞内 ATP,ADP,AMP含量明显减低 ,细胞外 ADP,AMP含量明显增高 .结果提示氯丙烯可引起神经细胞内钙增加和细胞内线粒体 SDH活性降低 ,ATP合成减少 .Using nerve cells from chicken embryo,the allyl chloride- induced changes in Ca,P,ATP,ADP and AMP contents and succinate dehydrogenase(SDH) activi- ties were studied.The results showed that with allyl chloride concentration increased, the percentage content of Ca in nerve cells was increased significantly,however,the contents of P,ATP,ADP,AMP and the activities of SDH were markedly decreased. These suggest that the allyl chloride- in- duced calcium unhomeostasis in nerve cells be related to inhibition of mitochondrial SDH activity and the decreasing in ATP synthesis,leading to increase in intracellu- lar Ca2 +.Inhibition of energy synthesis in- duced by allyl chloride in nerve cells might be one of the mechanisms of calcium un- homeostasis.

关 键 词：氯丙烯 神经元 钙 琥珀酸脱氢酶 磷 神经细胞 

分 类 号：R135[医药卫生—劳动卫生]