环孢菌素A生产菌原生质体转化系统的建立  

Construction of Protoplasts Transformation System for Cyclosporin A Industrial Strain

作　　者：戴梦[1] 刘静[1] 赵颖[1] 张佳[1] 章丽[1] 郑桂珍[1] 

机构地区：[1]华北制药集团新药研究开发有限责任公司微生物药物国家工程研究中心,河北石家庄050015

出　　处：《化学与生物工程》2013年第3期36-38,共3页Chemistry & Bioengineering

基　　金：石家庄高端医药产业园创新药物孵化基地资助项目(2011ZX09401-306)

摘　　要：以腐草霉素抗性为选择标记,建立了环孢菌素A生产菌株的基因转化体系,成功将外源基因转入环孢菌素A生产菌,并整合到染色体上。传代实验表明,外源基因可以稳定表达。该体系的建立为环孢菌素A生产菌的基因工程育种奠定了基础。A protoplasts transformation system for high-productive industrial strain of cyclosporin A was successfully constructed by using phleomyein resistant gene as select marker. The PCR results proved that the foreign DNA had been integrated in the chromosome of T. infiatum and it could be expressed steadily after sev-eral generations. This system laid a foundation for genetic engineering breeding of the cyelosporin A-producing strain.

关 键 词：环孢菌素A 多孔木霉 原生质体转化 

分 类 号：Q813[生物学—生物工程]