Q Sepharose^(TM)-XL强阴离子交换色谱快速纯化精氨酸激酶及其多克隆抗体的研究  

Study on Fast Purification of Arginine Kinase and Its Polyclonal Antibody via Q Sepharose^(TM)-XL Strong Anion Exchange Chromatography

作　　者：陈婷[1] 吴继美[1] 李任强[1] 

机构地区：[1]暨南大学生物工程学系,广东广州510632

出　　处：《化学与生物工程》2013年第3期67-70,81,共5页Chemistry & Bioengineering

摘　　要：利用Q SepharoseTM-XL强阴离子交换色谱从虾的精氨酸激酶粗提液中纯化出精氨酸激酶,以其免疫大白兔,然后利用Q SepharoseTM-XL强阴离子交换色谱从高免疫兔血清中纯化精氨酸激酶的多克隆抗体。采用SDS-PAGE检测精氨酸激酶及其抗体的纯度,并进行了活性测定。结果表明,利用Q SepharoseTM-XL强阴离子交换色谱纯化出的精氨酸激酶及其多克隆抗体纯度高、活性强。建立了快速高效、操作方便而又适合实际应用的制备精氨酸激酶及其多克隆抗体的方法,可为精氨酸激酶及食品过敏的相关研究提供帮助。Q Sepharose^TM-XL strong anion exchange chromatography was used to purify shrimp arginine ki- nase from arginine kinase crude extract. After the white rabbit was immunized using arginine kinase,the hyper-immune sera was collected and Q Sepharose^TM-XL strong anion exchange chromatography was again used to pu-rify arginine kinase polyclonal antibody from these sera. The high purity and activity of arginine kinase and its polyclonal antibody purified by Q Sepharose^TM-XL strong anion exchange chromatography had been proved by SDS-PAGE. Laboratory method for fast and efficiently purification of arginine kinase and its polyclonal antibody has been built,which would be helpful for arginine kinase study and food allergies research.

关 键 词：Q SepharoseTM-XL强阴离子交换色谱 精氨酸激酶 多克隆抗体 纯化 

分 类 号：TQ464.7[化学工程—制药化工]