慢性丙肝患者血清中自身抗体的检测及分析  

Detection and analysis of antibodies in serum of patients with chronic HCV

作　　者：叶国强[1] 

机构地区：[1]广东省深圳市宝安区人民医院检验科,广东深圳518000

出　　处：《中国医学工程》2013年第2期28-29,共2页China Medical Engineering

摘　　要：目的分析慢性丙型肝炎(CHC)患者血清中自身抗体阳性率及其临床意义。方法选取2009年9月-2012年9月于我院就诊的200例CHC患者,采取两种方法即ELISA(酶联免疫吸附试验)和LIA(免疫印迹法)检测患者血清中的自身抗体,检测自身抗体(ANA、ASA、AMA、RF、LKM-I)阳性率,并比较自身抗体(+)及自身抗体(-)患者临床特征。结果CHC患者血清中自身抗体至少一种阳性的70例,阳性率35.0%。ANA阳性率最高,为18.0%;其次为RF阳性率(12.5%),再次为AMA阳性率(3.0%),LC-1及LKM-1阳性率为1.5%和2.55。ELISA组对自身抗体的检出率明显低于LIA组(25.0%vs 35.0%,P<0.05);另外,自身抗体阳性组患者的肝脏功能差,免疫功能异常,病毒复制活跃。结论丙型肝炎病毒(HCV)感染者存在自身免疫反应。自身抗体可能是HCV感染后肝组织损伤的重要因素。[ Objective ] To explore diagnosis value on their own antibody detection of chronic HCV from the cases retrospectively analyzed. [ Methods ] Select in our clinic 200 cases （September 2009 to September 2012） patients with case-control analysis, patients were taken to the hospital after two methods namely ELISA （enzyme-linked immunosorbent assay） and LIA （Western blotting） detection in the serum of patients with their own antibody, this paper discusses two methods of detecting level and at the same time through own antibody testing results were analyzed and discussed its for chronic hepatitis c treatment and prognostic value. [ Resluts ] significantly lower than the LIA group, numerical were35.0% statistical significance （p 〈 0.05）; Autoantibody ELISA group for their own antibody detection rate was and 25.0% respectively, significant difference, there is to the prognosis of chronic hepatitis c is of guiding significance, especially for the prediction of liver cirrhosis. [ Conclusion ] Chronic hepatitis c patients autoantibody detection application LIA method more accurate, testing positive rate is high, autoantibody positive diagnosis of liver cirrhosis diagnosis have clear value, can carry out in clinical use.

关 键 词：慢性丙型肝炎 自身抗体 价值分析 

分 类 号：R512.63[医药卫生—内科学]