当归补血汤中黄芪的鉴别及黄芪甲苷的含量测定  

The Identification of Radix-Astragali and Determination of the Quantify of Astragaloside Ⅳ in Dangguibuxue Decoction

作　　者：张凤瑞[1] 周贤[1] 刘炎[1] 翁丽丽[1] 王淑敏[1] 韩雪华[2] 

机构地区：[1]长春中医药大学,吉林长春130117 [2]延边大学,吉林延吉133000

出　　处：《吉林中医药》2013年第3期289-291,共3页Jilin Journal of Chinese Medicine

基　　金：吉林省科技厅自然科学基金项目(编号:201105058)

摘　　要：目的:建立当归补血汤中黄芪的鉴别和黄芪甲苷的含量测定方法,为当归补血汤质量标准的建立奠定基础。方法:采用薄层色谱法定性鉴别当归补血汤中的黄芪药材,并利用高效液相色谱法测定黄芪甲苷含量。色谱柱:Diamonsil ODS-C18(5μ,250 mm×4.6 mm)分析柱;流动相:乙腈-水(32∶68);流速:0.6 mL/min;柱温:35℃;载气流速:2.7 mL/min;ELSD检测器漂移管温度:100℃;进样量20μL。结果:黄芪的薄层色谱鉴别条件专属性好,高效液相色谱检测黄芪甲苷测定方法具有较好的准确性、重现性和可行性,样品在10.01～20.2μg范围内呈良好的线性关系,平均加样回收率为95.04%,RSD=0.61%。结论:所建立方法简便、准确,高效液相色谱分离效果好、线性范围宽、灵敏度高,可用于本品的质量控制。Objective： To establish the method for identifying Radix Astragali and detecting the quantity of Astragaloside IV- in Dangguibuxue Decoction and lay the foundation for the establishment of Dangguibuxue Decoction quality standard. Methods：Identify Radix Astragali qualitatively by thin layer chromatography（TLC） and detect the quantity of Astragaloside IV in Dangguibuxue Decoction by High performance liquid chromatography（HPLC）. Using Diamonsil ODS-Cjs （5 tL, 250 mm x 4.6 mm） analytical column; the mobile phase was composed of a mixture of acetonitrile-water（32：68） ;the flow rate was 0.6 mL/min; the column temperature was 35 ℃ ;the flow rate of carrier gas was 2.7 mL/min; ELSD detector, drift temperature was 100 ℃ ; the sample size was 20 μL. Results： The TLC method of Radix Astragali is specific. HPLC method for de- tecting the quantity of Astragaloside IV has a good accuracy, reproducibility and feasibility. There was a good linear relationship between the absorption area value and the concentration in the range of 1.01-20.2μg for sample. The average recovery was 95.04% with the RSD of 0.61%. Conclusion：The method is simple and accurate,which has good separation efficiency, wide linear range and high sensitivity. The method can be used to control the quality of Dangguibuxue Decoction.

关 键 词：当归补血汤 高效液相色谱 黄芪 黄芪甲苷 

分 类 号：R284.2[医药卫生—中药学]