2010-2011年我院新生儿败血症病原茵分布及耐药性研究  

Distribution and antibiotics resistance of bacteria isolated from neonates with septicemia

作　　者：夏卫良[1] 华春珍[1] 朱银燕[1] 王琦婧[1] 徐立群[2] 黄邢邢[1] 

机构地区：[1]杭州师范大学附属医院儿科,310018 [2]杭州师范大学附属医院细菌室,310018

出　　处：《浙江医学》2013年第3期209-211,231,共4页Zhejiang Medical Journal

摘　　要：目的探讨新生儿败血症的病原菌分布及其耐药性，为临床经验性诊治提供科学依据。方法选取疑诊为新生儿败血症的住院患儿常规进行血培养。将系统提示阳性的血培养瓶及时取出，划种于固体培养基上，经细菌自动鉴定系统（Vitek2-com-pact）进行菌种鉴定和药敏试验。结果确诊为新生儿败血症113例，共培养出细菌117株，血培养阳性率102％，革兰阳性菌923％，革兰阴性菌仅占77％。凝固酶阴’性葡萄球菌93株，占795％，其中表皮葡萄球菌和溶血葡萄球菌最多见，分别为49．6％和124％。葡萄球菌对苯唑西林的耐药率高达81％，所有阳性球菌均对万古霉素敏感，亚胺培南对革兰阴性杆菌保持较强抗菌活性。结论葡萄球菌是新生儿败血症的主要致病菌，且该菌对苯唑西林耐药率高，临床医师应根据药敏试验结果选择抗生素。Objective To investigate the distribution and antibiotics resistance of bacteria isolated from neonates with septicemia. Methods Blood samples from neonates suspected septicemia admitted from January 2010 to July 2011were col- lected and cultured. The blood samples with positive alarms in the Vitek system were inoculated on solid medium. Bacterial strains were identified and antibiotics susceptibility was tested with Vitek system. Results A total of 117 strains were isolated from 113 neonates with septicemia with a positive blood culture rate of 10.2%. The predominant strains （92.3%） were gram-posi- tive, while the gram-negative strains accounted for only 7.7%. Ninety-three isolates were coagulase-negative staphylococci and most of them were staphylococcus epidermidis （49.6%） followed by staphylococcus hemolyticus （12.4%） and 81% of the staphy- lococcus strains were resistant to oxacilin. All gram-positive strains were susceptible to vancomycin and most of the gram-neg- ative bacteria were susceptible to imipenem. Conclusion Staphylococcus is the most common pathogen causing neonatal sep- ticemia, with a high rate of resistance to oxacilln.

关 键 词：新生儿 败血症 病原菌 耐药性 

分 类 号：R722.131[医药卫生—儿科]