人工养殖暹罗鳄血体内、外抗肿瘤活性研究  

Anticancer Activity of Crocodylus siamensis Blood in vitro and in vivo

作　　者：赓迪[1] 段玉龙[1] 徐晓琳[1] 王春梅[1] 崔小冬[1] 欧阳召[1] 陈斌[1] 

机构地区：[1]北京中医药大学中药学院生物制药系,北京100102

出　　处：《中药材》2012年第12期1901-1904,共4页Journal of Chinese Medicinal Materials

基　　金：教育部留学回国人员科研启动基金;北京中医药大学自主课题

摘　　要：目的:研究暹罗鳄血体外对4种肿瘤细胞株的抑制作用以及体内对移植性肿瘤S180荷瘤鼠的影响。方法:制备暹罗鳄鱼血清和血浆,体外利用MTT法测定样品抑制肿瘤细胞增殖活性。体内采用S180移植肿瘤模型小鼠口服给药12 d,观察小鼠的生长情况、肿瘤生长情况、体重、对脏体比的影响。结果:较高浓度的鳄鱼血清和血浆对HepG2、BGC823、HeLa细胞和SKOV3细胞有增殖抑制作用,鳄鱼全血有抑制荷瘤鼠肿瘤生长的作用,抑制率为57.55%,且能使荷瘤小鼠生存质量改善。结论:人工养殖暹罗鳄血具有抗肿瘤作用。Objective：To study the anticancer effects of the blood of Crocodylus siamensis in vitro and in vivo.Methods：The inhibitory effects of serum and plasma of Crocodylus siamensis on proliferation of HepG2,BGC823,HeLa and SKOV3 cell were measured by MTT assay.The mouse S180 tumor model was used to evaluate the anti-tumor effect in vivo.Results：High dosage serum and plasma of Crocodylus siamensis could inhibit the proliferation of HepG2,BGC823,HeLa and SKOV3 cell.The tumor inhibitory rate of high dosage blood of Crocodylus siamensis on S180 tumor was up to 57.55%.Conclusion：The blood of breeding Crocodylus siamensis has anticancer activity.

关 键 词：暹罗鳄血 肿瘤细胞 S180小鼠 抗肿瘤活性 

分 类 号：R285.5[医药卫生—中药学]