护骨胶囊中5种成分HPLC同时测定方法的建立(英文)  

Simultaneous Determination of 5 Major Bioactive Components in Hugu Capsule by HPLC-DAD

作　　者：宋丽军[1] 赵文昌[1] 曾秋玲[1] 吴劲东[1] 李宝红[1] 

机构地区：[1]广东医学院药学院,广东东莞523808

出　　处：《中药材》2012年第12期2019-2023,共5页Journal of Chinese Medicinal Materials

基　　金：广东省科技计划项目(2010B030700073);湛江市科技攻关项目(2010C3101013);广东医学院博士启动项目(KY1004);广东省中医药管理局项目(2010192)

摘　　要：目的:建立护骨胶囊多成分定性、定量的分析方法,为护骨胶囊质量控制、药理药效研究提供依据。方法:采用采用高效液相色谱法,Kromasil C18色谱柱,流动相为甲醇-乙腈-1%冰醋酸,梯度洗脱,检测波长270、283、320和325 nm,流速:0.8 mL/min,柱温:30℃,对护骨胶囊中的主要成分绿原酸、2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷、阿魏酸、柚皮苷、淫羊藿苷进行定性、定量测定。结果:所建方法可用于对绿原酸等5种成分同时进行定性分析、定量测定。5种成分在线性范围内线性均良好(r>0.9992);精密度、稳定性、重复性(以相对标准偏差表示)均小于5%,加样回收率的范围为97.4%～99.4%,RSD值均小于1.72%。结论:首次建立了HPLC同时检测护骨胶囊多成分定性、定量的新方法。该方法简单、可行,可提高护骨胶囊的质量控制水平。Objective：To develop a qualitative and quantitative determination of multiple components for quality control of Hugu Capsule,a composite prescription in TCM.Methods：HPLC analysis was performed on a Kromasil C18 column（5.0 μm,250 mm × 4.6 mm） by gradient elution with methanol-acetonitrile-1% glacial acetic acid as the mobile phase at a flow-rate of 0.8 mL/min,the detection wavelengths were set at 270,283,320 and 325 nm at the temperature of 30 ℃.Results： Five components：chlorogenic acid,tetrahydroxy stilbene glucoside,ferulaic acid,naringin and icariin were simultaneously analyzed in this study.The calibration curves were exhibited linear regressions of at least r 0.9992.The injection precision,the intra-day precisions and the analysis repeatability were evaluated with the RSD values were all less than 5%.The mean recoveries of the five components were ranged from 97.4% to 99.4%,and RSD values all were less than 1.72%.Conclusion：This method is found to be convenient,fast,accurate,and is applicable to analyze the multi-constituents in Hugu Capsule.

关 键 词：高效液相色谱 护骨胶囊 定性分析 定量分析 

分 类 号：R286.1[医药卫生—中药学]