新城疫病毒长春株F基因和HN基因的序列分析  

Comparison of Homology of F Gene and HN Gene Sequences of Changchun Strains NDV

作　　者：金扩世[1] 金宁一[1] 王兴龙[1] 丁壮[1] 郭志儒[1] 王宏伟[1] 殷震[1] 

机构地区：[1]解放军军需大学研究所,吉林长春130062

出　　处：《动物科学与动物医学》2000年第3期38-39,共2页Animal Science & Veterinary Medicine

基　　金：国家自然科学基金资助项目! (3 9893 2 90 - 4 - 3 );吉林省科委资助项目! (96 3 5 0 6 )

摘　　要：从长春地区分离到一株新城疫病毒 ,经过对其进行生物学研究表明该毒株具有新城疫病毒弱毒株的一些特性。本试验通过 RT-PCR方法测定出该毒株 F基因的核苷酸序列为 1758bp,编码有 53 3个氨基酸残基 ;HN基因核苷酸序列为 173 1bp,编码 577个氨基酸残基。与国外发表的部分新城疫病毒强毒株和弱毒株之间相同序列进行比较 ,F基因核苷酸序列的同源性在 88.2 %～ 96.7%之间 ,氨基酸序列的同源性在 90 .1%～ 98.1%之间 ;HN基因核苷酸序列的同源性在 88.4 %～ 98.1%之间 ,氨基酸序列的同源性在 91.2 %～ 98.1%之间。 F蛋白裂解位点区 ( 112～ 117)氨基酸组成与弱毒株一致 。This test amplified F protein and HN protein of Changchun strain NDV by TR PCR and sequenced.The nucleotide sequences of the F protein were 1 758 bp,encoding 553 amino acid residues.The nucleotide sequences of HN protein were 1 731 bp,encoding 577 amino acid residues.Compared with the published virulent strains and avirulent strains of NDV,the homology of nucleotied and amino acid sequences of F protein were 88.2%～96.7% and 90.1%～98.1% separately.The homology of nucleotide sequences and amino acid sequences of HN protein were 88.4%～98.1% and 91.2～98.1% separately.The amino acid sequences of cleavage site region (112～117) were similar to that of all other avirulent strains.The results showed that the changchun strain was a avinuent strain of NDV.

关 键 词：新城疫病毒 F基因 HN基因 同源性比较 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]