RP-HPLC法测定心肌安颗粒中绿原酸和芍药苷  

Contents determination of chlorogenic acid and paeoniflorin in Xinji'an granules by RP-HPLC

作　　者：张衍秀[1] 陈新[1] 关枫[1] 

机构地区：[1]黑龙江中医药大学药学院,哈尔滨150040

出　　处：《哈尔滨商业大学学报（自然科学版）》2012年第6期647-649,共3页Journal of Harbin University of Commerce：Natural Sciences Edition

摘　　要：建立RP-HPLC同时测定心肌安颗粒剂中含绿原酸、芍药苷两种有效成分的方法.采用Dia-monsil C18(4.6 mm×250 mm,5μm)色谱柱,以乙腈-0.2%磷酸水为流动相梯度洗脱,流速1.0mL/min,柱温35℃,检测波长230 nm.绿原酸和芍药苷质量浓度分别在20～120μg/mL(r=0.999 9)和30～180μg/mL(r=0.999 8)范围内与峰面积线性关系良好,绿原酸和芍药苷平均加样回收率(n=6)分别为99.38%(RSD=0.96%)和99.91%(RSD=0.82%).三批制剂测定结果显示,含绿原酸量为10.36 mg/g(RSD=0.73%),含芍药苷量为8.18 mg/g(RSD=0.58%).本方法快速、准确,重复性好,可用于同时测定心肌安颗粒剂中绿原酸和含芍药苷的量.This paper established a method to detect the content of chlorogenic acid and paeoniflorin in Xinji＇an Granules by RP-HPLC at the same time.The chromatographic conditions were as follows： with the diamonsil C18（4.6 mm ×250 mm,5 μm） as the chromatographic column,acetonitrile-0.2% phosphoric acid solution as the mobile phase（grades elution）,1.0 mL/min as the flow rate,35 ℃ as the column temperature,λ=230 nm.The linear range for chlorogenic acid and paeoniflorin were 20~120 μg/mL（r=0.999 9） and 30~180 μg/mL（r=0.999 8）.The average recovery for chlorogenic acid and paeoniflorin（n=6）were 99.38%（RSD=0.96%） and 99.91%（RSD=0.82%）.The chlorogenic acid and paeoniflorin were 10.36 mg/g（RSD=0.73%） and 8.18 mg/g（RSD=0.58%） determined by the method applied.The method was simple,accurate,and could be used for the quality control of this preparation.

关 键 词：心肌安颗粒剂 绿原酸 芍药苷 RP-HPLC 

分 类 号：R284[医药卫生—中药学]