慢性应激抑郁型黄褐斑动物模型建立与现有模型的比较研究  

Establishment of Chloasma Guinea pig Model with chronic unpredictable mild stress depression and comparative study of it with existent models

作　　者：杨鹏[1] 杨慧兰[2] 麦跃[1] 戴勇[3] 孙林潮[1] 鲁东平[4] 

机构地区：[1]大连医科大学附属深圳阳光医院皮肤科,广东深圳518000 [2]南方医科大学附属广州军区广州总医院皮肤科,广东广州510010 [3]暨南大学医学院第二附属深圳市人民医院动物实验中心 [4]深圳市宝安区慢性病防治院

出　　处：《中国美容医学》2013年第3期349-354,共6页Chinese Journal of Aesthetic Medicine

基　　金：2010年广东省深圳市科学技术计划项目基金资助(深圳市科技计划项目编号:201003464)

摘　　要：目的:建立并验证慢性应激抑郁型黄褐斑动物模型,并与现有其他模型进行比较。方法:在注射黄体酮同时,进行慢性轻度不可预见的应激刺激,并紫外线局部照射。结果:黄体酮+紫外线+慢性应激抑郁法造模较其他方法更能导致皮肤丙二醛(Malondialdehyde,MDA)升高和超氧化物歧化酶(Superoxidedismutase,SOD)降低以及皮肤黑素细胞的增加,并且具有抑郁症的行动学表现。与空白组比较,经慢性轻度不可预见性应激刺激的动物体重显著下降,敞箱试验中穿格次数、理毛时间和次数、直立次数得分显著下降,液体消耗试验中糖水消耗和糖水偏爱百分比明显下降,而纯水消耗显著提高。结论:黄体酮+紫外线+慢性应激抑郁法建立的抑郁型黄褐斑豚鼠多因素模型获得成功。与现有其他模型比较,其皮肤MDA、SOD与皮肤黑素细胞等客观指标的变化也更加接近人类黄褐斑的病变。Objective To establish and authenticate the Chloasma Guinea pig Model with chronic unpredictable mild stress depression and comparative study of it with existent models. Methods After the injection of progesterone,chronic unpredictable sequence of mild stressors stimulated them and gave them partially ultraviolet radiation. Results The Guinea pig model of depression had been established successfully at 28th day.Multiple models of progesterone injection and ultraviolet radiation compounding with chronic unpredictable sequence of mild stressors can heighten MDA,depress SOD,and increase melanocytes of skin. Meanwhile,the ethology of this model showed it＇s syndrome of Depressive position.The guinea pig showed reduced squares crossing and rearing in open-field test, a significantly reduced consumption of preference for sucrose solutions and an increased pure water consumption as compared with control group. Conclusion The multiple factor Chloasma model of chronic unpredictable mild stress depression has been built successfully. Compared with other existing models of MDA,SOD and skin melanocytes objective indicators of change closer to human Chloasma lesions.

关 键 词：黄褐斑 抑郁症 动物模型 

分 类 号：R758.42[医药卫生—皮肤病学与性病学]