早孕母胎界面固有模式识别受体NOD1/NOD2的表达  

Expression of Innate Pattern Recognition Receptors NOD1/NOD2 at Materno-Fetal Interface of Early Pregnancy

作　　者：聂晓露[1] 孟丽丽[1] 张媛媛[1] 陈慧[1] 王瞾华 陈欣[1] 张建平[1] 

机构地区：[1]中山大学孙逸仙纪念医院产科,广东广州510120

出　　处：《中山大学学报（医学科学版）》2013年第1期11-15,共5页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：国家自然科学基金(81270754;81170625;81000259;30973207;81070746);广东省自然基金(10151008901000007;10451008901004246);教育部博士点新教师基金(20090171120075)

摘　　要：【目的】明确早期妊娠母胎界面中固有模式识别受体NOD1/NOD2的表达情况。【方法】分别用免疫组织化学法及Real-Time PCR法检测12例早孕期绒毛及蜕膜组织中NOD1/NOD2表达。【结果】免疫组织化学结果显示绒毛组织中NOD1/NOD2主要定位于绒毛滋养细胞胞质中,蜕膜组织也检测到NOD1/NOD2分子的表达。在mRNA水平上,绒毛组织中NOD1/NOD2的表达量高于蜕膜基质细胞(DSC),分别为P=0.03和P=0.009;DSC中NOD1的表达量高于NOD2,P=0.029,差异具有显著性。绒毛组织中NOD2的表达高于NOD1,差异无统计学意义(P>0.05)。【结论】NOD1/NOD2在早孕期母胎界面中绒毛组织有表达,可能参与胚泡的着床。[Objective] To characterize the expression of NOD1/NOD2 at materno-fetal interface. [Methods] NOD1 and NOD2 expression by villi and decidua were evaluated by immunohistochemistry and real-time PCR（n = 12）. [ Results ] Immunohistochemistry result showed both NOD1 and NOD2 were expressed by villi and decidua at materno-fetal interface,and localized to cytoplasm of trophoblast cells and DSC. On the mRNA level, villi expressed more NOD1/NOD2 than decidual stromal cells （DSC, P = 0.03 and P = 0.009）. DSC expressed more NOD1 than NOD2, P = 0.029. The differences were significant. Villi expressed more NOD2 than NOD1, but the difference was not significant （P 〉 0.05）. [ Conclusion ] Villi and DSC express NOD1/NOD2 at materno-fetal interface of early pregnancy. NOD 1/NOD2 maybe participate in plantation of embryo.

关 键 词：母胎界面 固有免疫 NOD1 NOD2 

分 类 号：R71[医药卫生—妇产科学]