手术创伤对老龄大鼠海马炎症介质表达的影响  

Effects of Surgical Trauma on the Expression of Inflammatory Mediators in the Hippocampus of Aged Rats

作　　者：姚星[1] 彭勉[1] 鲁胜强[1] 王焱林[1] 

机构地区：[1]武汉大学中南医院麻醉科,湖北武汉430071

出　　处：《武汉大学学报（医学版）》2013年第2期171-173,共3页Medical Journal of Wuhan University

基　　金：国家自然科学基金资助项目(编号:30901393)

摘　　要：目的:探讨手术创伤对老龄大鼠海马肿瘤坏死因子α(TNF-α)、白介素1β(IL-1β)、诱导型一氧化氮合酶(iNOS)以及一氧化氮(NO)表达水平的影响。方法:健康雄性SD大鼠45只,月龄18月,体重500-600g,随机分成3组(n=15):对照组(C组)腹腔注射生理盐水3ml;麻醉组(A组)腹腔注射1%戊巴比妥钠注射液50mg/kg(稀释至3ml)麻醉,不行手术;手术组(S组)腹腔注射1%戊巴比妥钠注射液50mg/kg(稀释至3ml)麻醉后,行腹腔探查+阑尾切除+脾切除术。于麻醉或术后1,3,7d(T1,3,7)采用ELISA法测定海马TNF-α和IL-1β的表达,采用NO和NOS试剂盒测定海马组织NO和iNOS表达水平。结果:与C组和A组比较,在T1和T3时点,S组大鼠海马TNF-α、IL-1β蛋白水平以及iNOS和NO的表达上调(P<0.05),C组与A组上述指标差异无统计学意义(P>0.05)。结论:手术创伤导致的海马TNF-α、IL-1β及iNOS、NO的表达上调,可能与老龄大鼠术后认知功能障碍有关。Objective： To investigate the effects of surgical trauma on the expression of TNF-α, IL-1β, nitric ox- ide （NO） and inducible nitric oxide synthase （iNOS） in the hippocampus of aged rats. Methods. Forty- five 18-month-old male SD rats weighing 500-600 g were randomly divided into 3 groups （n= 15 in each group） ： control group （group C）, anesthesia group （group A）, and surgical group （group S）. Rats in groups C received saline 3 ml intraperitoneally. Anesthesia was induced by intraperitoneal 1 pentobarbital sodium 50 mg/kg in group A and S. The animals underwent appendectomy and splenectomy under anesthesia in group S. The hippocampi were removed for determination of the expression of TNF-α, IL-1β, NO, and iNOS. Results： In group S, the expression of TNF-α, IL- 1β, NO, and iNOS in hippocampus were increased on the 1st and 3rd days after operation as com- pared with groups C and A （P〈0.05）. There was no significant difference in variables mentioned above between groups C and A（P〉0.05）. Conclusion： The up-regulations of the expressions of inflam- matory mediators in the hippocampus may play a role in postoperative cognitive dysfunction.

关 键 词：外科手术 炎症 一氧化氮合酶 海马 老龄鼠 

分 类 号：R614.2[医药卫生—麻醉学]