慢性阻塞性肺疾病患者外周血单个核细胞CXCR3的表达及与气流受限的关系  

Correlation Between the Expression of CXCR3 in Peripheral Blood Mononuclear Cells and the Airflow Limitation in Patients with Chronic Obstructive Pulmonary Disease

作　　者：聂莉[1] 李婷[1] 刘淑华[1] 刘以鹏[1] 

机构地区：[1]武汉大学人民医院老年病科,湖北武汉430060

出　　处：《武汉大学学报（医学版）》2013年第2期241-244,共4页Medical Journal of Wuhan University

基　　金：中央高校基本科研业务费专项资金青年教师资助项目(编号:4101027)

摘　　要：目的:探讨CXCR3在慢性阻塞性肺疾病(COPD)发病机制中的作用及其与COPD患者气流受限的关系。方法:选择COPD急性加重期(AECOPD)、稳定期患者和正常对照组各30例,采用流式细胞术检测外周血T细胞亚群CD8+T细胞和CD4+T细胞数,酶联免疫吸附试验检测外周血单个核细胞(PMBCs)CXC趋化因子受体3(CXCR3)和干扰素-γ诱导蛋白-10(CXCL10)的表达。结果:流式细胞术显示,AECOPD患者外周血中CD8+T细胞数较COPD稳定期组及对照组明显增高(P<0.05);且COPD稳定期组较对照组明显升高(P<0.05)。在蛋白水平,与AECOPD组相比,COPD稳定期组和对照组PMBCs中CXCR3和CXCL10的表达均明显降低(P<0.05)。与正常组相比,COPD稳定期组PMBCs中CXCR3的表达显著上调(P<0.05)。PMBCs中CXCR3及CXCL10的表达与FEV1/FEV%、FEV1占预计值%均呈负相关(P均<0.05)。结论:CXCR3及其配体CXCL10参与COPD气道炎症的发生与发展,与COPD患者气流受限呈正相关。Objective： To explore the roles of CXCR3 in the pathogenesis of chronic obstructive pulmo- nary disease （COPD） and the relationship with the airflow limitation in COPD patients. Methods： Thirty patients with COPD during acute exacerbation （AECOPD）, 30 patients with COPD stable period, and 30 normal subjects （control group） were chosen. The T-lymphocyte subset （CD4＋ , CD8＋） of peripheral blood was measured by flow cytometry. Expression of CXCR3 and interfer- on-y induced protein-10 （CXCL10） in the PBMCs at protein levels were detected by ELISA. Results. Flow cytometry indicated that CD8＋ T ceils in peripheral blood of the patients with AE- COPD were significantly increased as compared with those of the patients with COPD stable peri- od and the control group （P〈0.05）. While CD8＋ T cells in the patients with COPD stable period was significantly increased as compared to the control group （P〈0.05）. At the protein level, there were significantly higher levels of CXCR3 and CXCL10 on the PBMCs of the patients withAECOPD as compared to the patients with COPD stable period and the control group （P〈0.05）. The expression of CXCR3 in the control group were significant lower than that in the patients with COPD stable period （P〈0.05）. The level of CXCR3 and CXCL10 showed a positive corre- lation with the level of FEV1/FEVG and FEVL1% predicted data （P〈0.05）. Conclusion. CXCR3 and CXCL10 participate in the occurrence and development of COPD, and correlate positively to the degree of airflow limitation in COPD patients.

关 键 词：慢性阻塞性肺疾病 外周血单个核细胞 CXCR3 气流受限 

分 类 号：R563.9[医药卫生—呼吸系统]