高效液相色谱法测定福多司坦原料药的含量  

Determination of the content of active pharmaceutical ingredients from Fudosteine by HPLC

作　　者：武晓红[1] 赵辉[1] 肖娴[1] 

机构地区：[1]河南大学药学院,河南开封475004

出　　处：《中国医药科学》2013年第4期103-104,共2页China Medicine And Pharmacy

摘　　要：目的建立福多司坦原料药含量的测定方法。方法采用高效液相色谱法,以十八烷基硅烷键合硅胶柱C18(250mm×4.6mm,5mm)为色谱柱,水-甲醇(99.5:0.5)为流动相,流速为0.8mL/min,检测波长:220nm,温度:30℃。结果福多司坦的线性范围为y=1575.2x-1319,r=0.989242,平均回收率为95.89%(RSD=1.7%)。结论该法精确可靠,简单方便,建立了HPLC法测定福多司坦原料药中的福多司坦含量的方法。Objective To establish the method for the content determination of active pharmaceutical ingredients in fudosteine. Methods HPLC was used with C18 （250 mm×4.6 mm,5 mm） column,the mobile phase consisted of H20-CH3OH（99.5 ： 0.5）.Wavelength for detection was 220 nm,and the flow rate was 0.8 mL/min. Results The range of calibration curve was y=1 575.2x-1 319,r=0.989 242,the method recovery of fudosteine tablets was 95.89%（RSD=1.7%）. Conclusion The method is sensitive, accurate, and can control the quality of fudosteine.

关 键 词：福多司坦 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]