荧光定量PCR检测HBV-DNA与ELISA法乙肝免疫学检测的探讨  

Study of fluorescence quantitative PCR detection of HBV-DNA and immunological detection of hepatitis B by ELISA

作　　者：黄静[1] 

机构地区：[1]四川省凉山彝族自治州第一人民医院检验科,四川凉山615000

出　　处：《中国医药科学》2013年第6期110-111,123,共3页China Medicine And Pharmacy

摘　　要：目的探讨荧光定量PCR法检测HBV-DNA与ELISA法乙肝免疫学指标的相关性。方法采用ELISA法和荧光定量PCR技术分别对353例乙型肝炎患者血清进行免疫学指标的检测及HBV-DNA的定量检测。结果 ELISA法呈大三阳组患者的HBV-DNA阳性率及病毒平均拷贝数均高于小三阳组;HBeAg(+)组的阳性率及病毒平均拷贝数高于HBeAg(-)组;HBeAb(+)组有较低的HBV-DNA阳性率及病毒平均拷贝数。结论 HBV-DNA的含量与乙肝免疫学检测结果具有相关性。临床上应注意两者联合应用。Objective To explore the correlation between fluorescence quantitative PCR assay for detection of HBV-DNA and HBV Immunological Indexes in ELISA method. Methods Quantitative detection of HBV-DNA and immunological indexes were detected in 353 cases of hepatitis B patients by ELISA and fluorescence quantitative PCR Technology. Results The positive rate of HBV-DNA and virus copy number in the group of HBsAg （＋）/HBeAg （＋）/ HBcAb（＋） were higher than the average in the group of HBsAg（＋）/HBeAb（＋）/HBcAb（＋）,higher than that of HBeAg （-） group.And HBeAb（＋） group had a lower positive rate and lower copies of the virus. Conclusion The level of HBV- DNA had the correlation with immunological detection of hepatitis B. We should pay attention to the combination of them in clinical.

关 键 词：HBV—DNA ELISA法 乙肝 免疫标志物 

分 类 号：R512.62[医药卫生—内科学]