华蟾素注射液对与人淋巴管内皮细胞共培养下人肝癌HepG-2细胞的增殖及侵袭的影响  

Effect of Cinobufacini Injection on proliferation and invasiveness of human hepatoma HepG-2 cells co-cultured with human lymphatic endothelial cells

作　　者：付海燕[1] 高山[1] 田莉莉[1] 陈秀英[1] 崔晓楠[1] 

机构地区：[1]大连医科大学第一附属医院肿瘤科,辽宁大连116011

出　　处：《中国临床药理学杂志》2013年第3期199-201,共3页The Chinese Journal of Clinical Pharmacology

基　　金：国家自然科学基金资助项目(81173615)

摘　　要：目的观察华蟾素注射液对与人淋巴管内皮细胞(HLEC)共培养下HepG-2细胞增殖、异质黏附及侵袭的影响。方法用Transwell法建立HepG-2细胞与HLEC共培养系统;用台盼蓝拒染法与MTT法观察华蟾素注射液对共培养系统中HepG-2细胞增殖、异质黏附力的影响;用Transwell侵袭小室观察华蟾素注射液对共培养系统中HepG-2细胞侵袭力的影响。结果华蟾素注射液能够明显抑制共培养系统中HepG-2细胞增殖、异质粘附及侵袭(均P<0.05),且呈剂量依赖性。结论华蟾素注射液能够抑制共培养系统中HepG-2细胞的增殖、黏附及侵袭。这可能是华蟾素注射液抑制肿瘤转移的机制之一。Objective To study the effect of Cinobufacini Injection on proliferation,heterogeneous adhesion and invasiveness of human hepatoma HepG-2 cells co-cultured with human lymphatic endothelial cells（HLEC）.Methods Co-culture system of human hepatoma HepG-2 cells and HLEC was established by means of Transwell chamber.Cell proliferation was analysed by Trypan blue stain assay.MTT assay was used to observe the heterogeneous adhesion capacity of HepG-2 cells co-cultured with HLEC.Transwell invasion chamber was used to observe the invasiveness capacity of HepG-2 cells co-cultured with HLEC.Results Cinobufacini Injection significantly inhibits proliferation,heterogeneous adhesion and invasiveness of hepG-2 cells co-cultured with HLEC in dose dependent ways（all P0.05）.Conclusion Cinobufacini Injection can inhibit the capability of proliferation,invasiveness and heterogeneous adhesion of HepG-2 cells,wich might contribute the inhibiting mechanisms of Cinobufacini Injection on tumor metastasis.

关 键 词：华蟾素注射液 HEPG-2细胞 人淋巴管内皮细胞 增殖 异质粘附 侵袭 

分 类 号：R285.6[医药卫生—中药学] R979.1[医药卫生—中医学]