洛伐他丁对人脐静脉内皮细胞Toll样受体2蛋白及mRNA表达的影响  

Effects of lovastatin on the protein and mRNA expressions of Toll-like receptor 2 in the human umbilical vein endothelial cells

作　　者：王洋[1] 

机构地区：[1]辽宁医学院附属第一医院心脏大血管外科,辽宁锦州121001

出　　处：《中国临床药理学杂志》2013年第3期202-204,共3页The Chinese Journal of Clinical Pharmacology

摘　　要：目的研究洛伐他丁对Toll样受体2(TLR2)及下游信号转导通路中主要元件髓样分化因子88(MyD88)表达的影响。方法体外培养人脐静脉内皮细胞(HUVEC),用TLR2特异性配体脂磷壁酸(LTA)刺激HUVEC细胞,并加入洛伐他丁干预24 h,收集各组细胞,用RT-PCR和Western Blot方法分别检测TLR2和MyD88基因和蛋白表达水平。结果 LTA能够增加HUVEC TLR2和MyD88 mRNA以及蛋白表达水平(P<0.05),洛伐他丁干预后明显抑制LTA对TLR2和MyD88 mRNA以及蛋白表达的增强作用。结论洛伐他丁发挥抗炎作用可能是通过对TLR2及下游MyD88依赖的信号转导抑制作用而实现,这可能是其抗动脉粥样硬化的机制之一。Objective To investigate the anti-atherosclerotic effects of lovastatin on the expression of TLR2 and its downstream signal transduction pathway in human umbilical vein endothelial cells（HUVEC）.Methods Lipoteichoic acid（LTA） stimulated HUVECs were treated with lovastatin for 24 h.The mRNA expression of TLR2,MyD88 were determined by RT-PCR.Protein levels of them were also measured by Western blot.Results Both mRNA and protein expressions of TLR2 and MyD88 were increased significantly in the LTA-treated cells and were obviously decreased after lovastatin treatment.Conclusion Lovastatin can reduce the expressions of TLR2 and MyD88 at mRNA and protein levels.The lovastatin may exert the function of anti-artherosclerosis through blocking the TLR2 and MyD88 expressions.

关 键 词：洛伐他丁 抗动脉粥样硬化 人脐静脉内皮细胞 

分 类 号：R966[医药卫生—药理学] R972.6[医药卫生—药学]