五味子含药血清中木脂素类成分的UPLC-MS/MS分析  

Analysis of lignans in serum containing drug of Schisandra chinensis by UPLC-MS/MS

作　　者：窦志华[1] 罗琳[2] 陈洁玉[1] 安莉萍[3] 杨爱华[3] 

机构地区：[1]南通大学附属南通第三医院药学部,江苏南通226006 [2]南通大学药学院,江苏南通226001 [3]南京中医药大学药学院,南京210046

出　　处：《中国临床药理学杂志》2013年第3期215-218,共4页The Chinese Journal of Clinical Pharmacology

基　　金：江苏省南通市应用研究计划基金资助项目(K2008027)

摘　　要：目的分析五味子含药血清中的木脂素类成分。方法 UPLC-MS/MS检测,经与五味子提取物、空白血清总离子流色谱图比较,发现含药血清中的药源性成分;经与对照品的保留时间和质谱数据相比对,对部分原形成分进行鉴定;经与提取物相同保留时间色谱峰质谱数据比较并参考相关文献,对部分原形成分进行推测。结果大鼠灌胃给予五味子提取物后,含药血清中产生了药源性成分,包括代谢产物和原形成分。鉴定了其中4个原形成分:五味子醇甲、五味子醇乙、五味子甲素和五味子乙素;推测了另外10个原形成分:戈米辛D、巴豆酰戈米辛H、当归酰戈米辛H、当归酰戈米辛P、五味子酯乙、五味子酚、戈米辛O、戈米辛N、6-O-苯甲酰戈米辛O和五味子丙素,所有14个成分均为联苯环辛烯类木脂素。结论五味子含药血清中的药源性成分主要为联苯环辛烯类木脂素。Objective To analyze lignans in serum containing drug（SCD） of Schisandra chinensis.Methods Samples was detected by UPLC-MS/MS.By comparing the total ion current（TIC） chromatogram of SCD with the ones of extract of Schisandra chinensis and blank serum,drug-induced constituents were found.Some original compounds were identified by comparing the retention time and mass data with reference compounds and some other ones were suspected by comparing the mass data of SCD with the ones of extract of Schisandra chinensis and consultting literatures.Results Drug-induced constituents were found in rat＇s serum after oral administration of Schisandra chinensis,including original compounds contained in materia medica and their metabolites.Four original compounds in SCD were identified as schisandrin,schisandrol B,deoxyschisandrin,schisandrin B,and ten other ones were suspected as gomisin D,tigloylgomisin H,angeloylgomisin H,angeloylgomisin P,schisantherin B,schisanhenol,gomisin O,gomisin N,6-O-benzoylgomisin O,and schisandrin C.All fourteen ones were dibenzocyclooctadiene lignans.Conclusion Main drug-induced constituents in SCD of Schisandra chinensis were dibenzocyclooctadiene lignans.

关 键 词：五味子 含药血清 木脂素 超高效液相色谱-串联质谱 

分 类 号：R965.1[医药卫生—药理学] R977.9[医药卫生—药学]