滋肾活血法对糖尿病肾病大鼠肾组织Ⅳ-C及MMPs/TIMPs系统的影响  

Effects of method of nourishing kidney and promoting blood flow on expression of MMPs/TIMPs and Ⅳ-C in kidney of diabetic nephropathy rats

作　　者：邓顺有[1] 范萍[1] 张征 张彤[1] 吴琳英[1] 

机构地区：[1]广州医学院第一附属医院内分泌科,广东广州510120 [2]广州市海珠区第二人民医院内科,广东广州510250

出　　处：《中国现代医学杂志》2013年第2期36-41,共6页China Journal of Modern Medicine

基　　金：广州市中医药中西医结合科研基金(No:2010A22);广州医学院博士启动项目(No:2008C28)

摘　　要：目的观察活血降糖胶囊对糖尿病肾病(DN)大鼠肾组织Ⅳ型胶原(Ⅳ-C)蛋白表达及MMPs/TIMPs系统作用的影响。方法将SD雄性大鼠复制为DN动物模型,筛选DN成模大鼠,随机分成模型(DNM)组、中药(HX)组、西药(IR)组,并随机设正常对照(NC)组,分别予中西药物干预;8周后处死大鼠取肾组织,以免疫组织化学方法测定IV-C、MMP-9、MMP-2及TIMP-1、TIMP-2的蛋白表达,计算积分光密度及面密度比值。结果 HX组和IR组的Ⅳ-C免疫组织化学染色面密度及积分光密度较DNM组降低,MMP-9、MMP-2免疫组织化学染色面密度及积分光密度较DNM组升高,DNM组TIMP-1、TIMP-2与HX组和IR组比较呈强阳性表达,HX组和IR组染色面积及积分光密度均较DNM组降低,差异有统计学意义(P<0.01);MMPs/TIMPs比值与Ⅳ-C免疫组织化学染色面积呈负相关(r1=-0.929,r2=-0.908,P<0.01)。结论滋肾活血中药——活血降糖胶囊可能通过调节肾组织MMPs/TIMPs系统蛋白表达,改善Ⅳ-C代谢,延缓DN肾小球硬化进程,对DN肾脏发挥保护作用。[Objective] To investigate the expression of matrix metalloproteinases （MMPs）, tissue inhibitor of metalloproteinases （TIMPs） and collagen Ⅳ （IV-C） in kidney of diabetic nephropathy rats and the intervention effect of Huoxue Jiangtang capsule with nourishing kidney and promoting blood flow. [Methods] The male SD rats were intraperitoneally to reduce diabetic nephropathy animal models. The animal models were randomly divided into three groups： diabetic nephropathy model group （DNM）, medicated group treated by Huoxue Jiangtang capsule group （HX） and medicated group treated by irbesartan group （IR）, and randomly assigned normal control group （NC）. After 8 weeks the expression of MMPs, TIMPs and IV-C in rat model kidney tissue were detected by immunohistochemistry method （SP）. [Results] Compared with DNM group, HX group and IR group showed the values of area density and photodensity of positively stained area of MMP-2 and MMP-9 significantly increased while that of TIMP-2, TIMP-1 and IV-C reduced （P〈0.01）. The ratio of MMPs/TIMPs and photodensity of IV-C changed in reverse direction （r1=-0.929, r2=-0.908, P 〈0.01）.[Conclusion] Huoxue Jiangtang capsule with nourishing kidney and promoting blood flow may regulate the balance of MMPs and TIMPs and weaken the accumulation of Ⅳ-C to protect kidney and ameliorate the structure and function damage of diabetic nephropathy model rats.

关 键 词：糖尿病肾病 活血降糖胶囊 Ⅳ型胶原 MMPS TIMPS 

分 类 号：R587.2[医药卫生—内分泌]