芘对蚯蚓的生物有效性和抗氧化酶的影响  

BIOAVAILABILITY AND EFFECT OF PYRENE ON ANTIOXIDASES IN EARTHWORMS

作　　者：马丽丽[1] 张静[2] 李伟明[1] 李辉信[1] 

机构地区：[1]南京农业大学资源与环境科学学院,南京210095 [2]湖北理工学院矿区环境污染控制与修复重点实验室,湖北黄石435003

出　　处：《土壤学报》2013年第2期357-363,共7页Acta Pedologica Sinica

基　　金：国家自然科学基金项目(41101292);国家环境保护公益项目(201009032);江苏省教育厅的优势学科计划(PAPD)资助

摘　　要：以室内模拟试验研究了芘在土壤中的老化行为及其在蚯蚓体内的生物富集规律,及芘胁迫下蚯蚓体内抗氧化酶的变化规律。试验结果表明,芘的可提取态含量随其老化时间延长而呈现先快后慢的下降趋势。在不同浓度的芘污染土壤中,蚯蚓的生长抑制率在前60 d均大于对照,150 d时无显著差异(p<0.05)。蚯蚓体内芘浓度随土壤中芘浓度的增大而增加,但蚯蚓肠道中芘浓度均高于表皮,表明蚯蚓主要通过主动吞食土壤颗粒作用富集土壤中的芘而非表皮的被动穿透,然随着芘的老化,蚯蚓表皮和肠道中的芘浓度表现出下降规律,在前60 d和150 d表现出显著的差异(p<0.05)。蚯蚓体内超氧化物歧化酶(SOD)活性随着芘的老化,污染处理组的促进作用逐渐减弱,150 d时与对照相比,表现出显著抑制(p<0.05)。过氧化氢酶(CAT)活性在100 mg kg-1浓度的芘污染处理组中,前60 d被显著促进(p<0.05),150 d时无显著差异,而丙二醛(MDA)含量也随芘的老化而逐渐与对照表现出差异,150 d时50和100 mg kg-1浓度的芘污染组均表现出显著差异(p<0.05)。Aging behavior in soil, bioaccumulation and effect on antioxidases in earthworms of pyrene were investiga- ted in the lab. Results indicate that its aging rate was rather high at the initial stage, and then slowed down with the time passing by. Its effect inhibiting earthworms growth was rather significant in the first 60 d, but turned to be insignificant on the 150th day in all the pyrene treatments （p 〈 0.05）. The higher the pyrene concentration in the soil, the higher its con- centration in the earthworm. However, pyrene concentration was higher in earthworms＇ intestines than in the epidermis throughout the entire experiment, suggesting that earthworms accumulate pyrene mainly by ingesting it with soil particles rather than by pyrene penetrating through their epidermis. The concentration of pyrene in earthworms＇ epidermis and intes- tines decreased as the incubation went on, showing a significant difference between the determinations on the 60th day and the 150th day （p 〈 0.05 ）. The effect of pyrene promoting SOD activity in earthworm gradually weakened with pyrene ag- ing on, and turned to be inhibiting SOD activity on the 150th day （p 〈 0.05）. Compared with CK, Treatment Pyrene （ 100 mg kg-1） was significantly higher in CAT activity in earthworm in the first 60 days （p 〈 0. 05） , and was found al- most the same on the 150th day, while MDA content decreased with pyrene aging on and was found to be much lower on the 150th day in both Treatments Pyrene （50 and 100 mg kg-1） （p 〈0.05）.

关 键 词：芘 老化 蚯蚓 生物有效性 抗氧化酶 

分 类 号：S154.38[农业科学—土壤学]