新疆乌头属植物高效薄层色谱指纹图谱研究  

HPTLC Fingerprint Analysis of Aconitum Herbals in Xinjiang

作　　者：赵翡翠[1] 李杰[2] 刘淑敏[2] 聂继红[1] 

机构地区：[1]新疆医科大学附属中医医院,乌鲁木齐830000 [2]新疆医科大学中医学院,乌鲁木齐830011

出　　处：《中国药学杂志》2013年第7期512-517,共6页Chinese Pharmaceutical Journal

基　　金：国家自然科学基金资助项目(81060328)

摘　　要：目的建立新疆乌头属植物薄层色谱指纹图谱,并进行分析,评价其相似程度及亲缘性。方法采用高效硅胶G薄层板,以正己烷-乙酸乙酯-甲醇(6.4∶3.6∶1)为展开剂,以稀碘化铋钾溶液显色,获得其显色后的薄层指纹图谱,经ChemPattern化学指纹图谱分析系统解决方案软件生成共有模式,进行相似度分析、聚类分析和主成分分析。结果筛选和优化薄层色谱条件,斑点清晰,分离度好;建立了新疆乌头属植物薄层色谱指纹图谱共有模式,由6个特征峰组成。相似度分析结果表明,新疆乌头属植物不同种之间化学成分有一定差异,大致可分为3类。其中白喉乌头、空茎乌头与草乌、川乌相似度较高。结论建立的薄层色谱指纹图谱,为新疆乌头属药用植物指纹图谱的研究奠定了方法学基础,白喉乌头是与已有国家法定药材标准的川乌、草乌相似度较高、具有潜在药用价值的品种,值得进一步深入研究。OBJECTIVE To establish the HPTLC fingerprint of Aconitum plants in Xinjiang and to carry out similarity compari- son, cluster analysis and principal component analysis. METHODS Using high performance thin layer silica gel G plate, n-hexane- ethyl acetate-methanol（6. 4： 3.6：1 ）as developer, diluted bismuth potassium iodide solution as colour-developing agent, of the HPTLC fingerprints were obtained. The common pattern was explored by chemical fingerprint analysis system software. Similarity analysis, clus- ter analysis and principal component analysis were carried out. RESULTS The conditions for thin layer chromatography were opti- mized, the spots were clear, and the separation was good; similarity analysis showed that Xinjiang Aconitum had some differences in chemical composition between different plant species, which can be broadly divided into three categories. Aconitum leucostomum Wo- rosch, and Aconitum apetalum （Huth） B. Fedtsch. had high similarity with Aconitum carmichaelii Debx. and Aconitum kusnezoffii Reichb.. CONCLUSION The established thin-layer chromatographic fingerprints have laid the methodological foundation for the study of the fingerprint of Xinjiang Aconitum plants. Aconitum leucostomum Worosch. ,which is a widely distributed and abundantly re- served Aconitum species in Xinjiang, has potential medicinal value and similarity with Aconitum carmichaelii Debx. and Aconitum kus- nezoffii Reichb. Which have national quality standard, and is worthy of further studies.

关 键 词：新疆乌头属植物 薄层色谱指纹图谱 相似度分析 聚类分析 主成分分析 

分 类 号：R284[医药卫生—中药学]