原花青素对大鼠自身免疫性慢性非细菌性前列腺炎的治疗作用及其机制的研究  

Therapeutic Effect of Procyanidins on Chronic Abacterial Prostatitis in Rats and Its Mechanisms

作　　者：孙鑫波[1] 刘朝东[1] 王咸钟[1] 

机构地区：[1]重庆医科大学附属第一医院,重庆400016

出　　处：《中国药学杂志》2013年第7期521-527,共7页Chinese Pharmaceutical Journal

摘　　要：目的研究原花青素对前列腺炎的治疗作用及其作用机制。方法建立慢性非细菌性前列腺炎大鼠模型,动物随机分成对照组、模型组、原花青素高、中、低剂量组(50、25、10 mg.kg-1)。各组于第1、2、6、10天进行药物干预。光镜下观察各组前列腺组织病理学变化,测定前列腺组织和血浆中白细胞介素-10和巨噬细胞炎性蛋白-1α的表达,并测定超氧化物歧化酶活力和丙二醛水平。结果原花青素能有效改善慢性非细菌性前列腺炎造成的病理改变,以中、低剂量效果更佳。原花青素各剂量均能降低慢性非细菌性前列腺炎大鼠组织白细胞介素-10和巨噬细胞炎性蛋白-1α基因水平表达,并降低慢性非细菌性前列腺炎大鼠血浆白细胞介素-10和巨噬细胞炎性蛋白-1α蛋白水平表达。原花青素各剂量均能显著降低组织丙二醛的生成,但对超氧化物歧化酶活性没有显著影响。结论原花青素具有抗慢性非细菌性前列腺炎治疗效果,其机制可能部分与抗炎抗氧化有关。OBJECTIVE To study the therapeutic effect of procyanidin （PC） on rats with chronic abacterial prostatitis （CAP） and its underlying mechanisms. METHODS The experimental rat model of autoimmune nonbacterial prostatitis was established. The animals were randomly divided into control group, model group, PC high dose, medium dose and low dose group （50, 25 and 10 mg . kg - 1 ） , and received the corresponding treatment on day 1, 2, 6 and 10. A placebo normal control group was also set. HE stai- ning was used to observe the histopathological changes in prostate. The expressions of interleukin-10 （IL-10） and macrophage inflam- matory protein-1α （MIP-1α） in prostate tissue and plasma were detected. The activity of superoxide dismutase （SOD） and content of malondialdehyde （MDA） were observed. RESULTS The pathological change in CAP rats was improved after treating with PC, espe- cially at medium or low dose. All the three doses of PC could reduce the gene expressions of IL-10 and MIP-lct in prostate tissue and the protein expressions of IL-10 and MIP-1α in serum of CAP rats. The three doses of PC all decreased the content of MDA in prostate tissue of CAP rats, and showed no significant effect on the activity of SOD in tissue or plasma. CONCLUSION PC has potent therapeutic effect for chronic abacterial prostatitis, and its mechanism of action is partly related to its anti-inflammatory and anti-oxidation effect.

关 键 词：原花青素 慢性非细菌性前列腺炎 白细胞介素-10 巨噬细胞炎性蛋白-1Α 超氧化物歧化酶 丙二醛 

分 类 号：R697.33[医药卫生—泌尿科学]