一株副溶血弧菌噬菌体VPp1的分离鉴定及裂解性能  

Isolation, identification and lysis properties analysis of a Vibrio parahaemolyticus phage VPp1

作　　者：彭勇[1] 丁云娟[1] 林洪[1] 王静雪[1] 

机构地区：[1]中国海洋大学食品科学与工程学院食品安全实验室,山东青岛266003

出　　处：《海洋科学》2013年第1期96-101,共6页Marine Sciences

基　　金：国家自然科学基金资助项目(31071540);现代农业产业技术体系建设专项资金资助项目(nycytx-50);山东省自然科学基金资助项目(ZR2011CQ024)

摘　　要：为探究副溶血弧菌(Vibrio parahaemolyticus)的生物防治方法,从水产品市场处污水中分离出一株副溶血弧菌噬菌体VPp1。并借助噬菌斑形态、电镜、酶切等技术对其进行了分类鉴定,同时测定了其裂解谱、最佳感染复数、一步生长曲线以研究其裂解性能。分类鉴定结果表明,其核酸是线型双链DNA,大小在15 kb左右。具有一个正二十面体的头部,头部直径大约为44 nm,无尾,属盖噬菌体科(Tectivirus);裂解性能研究结果表明,其在双层平板上培养12 h可形成中心清亮的噬菌斑,周围有大而明显的晕环。最佳感染复数为0.0001,潜伏期为10 min,裂解量为90.3,是符合条件的潜伏期短裂解量大的理想噬菌体,可用作进一步的应用。In order to develop an effective bio-controlling method against Vibrio parahaemolyticus, a virulent phage VPp 1 was isolated from aquatic sewage water and identified by plaque shapes, restriction, electronic microscope and lysis properties analyses. The results revealed that VPpl contains a linear double stranded DNA with 15kb in size and it has an icosahedral head with 44 nm in diameter and does not have a tail, suggesting that VPpl belongs to the Tectivirus family. VPpl forms plaques with transparent center and large halo after culture in dou- ble-layer agar plate for 12 hours and its optimum MOI value was 0.0001. A one-step growth experiment showed that the latent period and burst size were estimated at 10 min and 90.3 phage particles/infected cell, respectively VPpl could be widely used because of its short latent period and large burst size

关 键 词：噬菌体 副溶血弧菌(Vibrio parahaemolyticus) 鉴定 裂解性能 

分 类 号：Q939.48[生物学—微生物学]