HPLC手性固定相法测定患者血浆中布洛芬对映体的浓度  

Determination of Ibuprofen Enantiomers in Plasma by HPLC-Chiral Stationary Phase

作　　者：施宪宝[1] 单丽娜[1] 郭斌[1] 周盾[1] 王桂军[1] 冯玉军 

机构地区：[1]辽宁医学院附属第一医院,辽宁锦州121001 [2]秦皇岛市妇幼保健院药剂科,河北秦皇岛066000

出　　处：《中国现代应用药学》2013年第3期316-318,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 建立用高效液相色谱法测定血浆中布洛芬对映体浓度的方法。方法 用正己烷-异丙醇混合液（100∶5）提取血浆样品中布洛芬，在Chirobiotic V（250 mm×4.6 mm，5 μm）柱上，以甲醇-冰醋酸-三乙胺（100∶0.04∶0.02）为流动相进行分离，于226 nm 波长处检测，内标为吲哚美辛。结果 R-布洛芬、S-布洛芬和内标没有干扰，分离度良好。R-布洛芬和S-布洛芬血药浓度在0.01~5 mg·L-1内线性关系良好。两个对映体相对回收率分别为99.1%~102.2%和99.3%~102.4%，绝对回收率分别为66.8~72.9和66.3~71.4，日内RSD分别为4.2%~5.4%和3.6%~5.1%，日间RSD分别为5.9%~7.3%和6.2%~7.9%。结论 该方法准确、快速、简便、灵敏度高、重复性好，可同时测量血浆中R-布洛芬和S-布洛芬的药物浓度，并可用于药动学参数的研究及患者血药浓度监测。OBJECTIVE To establish a method for the determination of plasma levels of enantiomers of ibuprofen. METHODS Ibuprofen was extracted from samples with Hexane-isopropanol（100∶5）. HPLC was performed on Chirobiotic column with methanol-aceticacid-triethylamine（100∶0.04∶0.02） as mobile phase. Detection wavelength was 226 nm. Indomethacin was used as the internal standard. RESULTS Under this chromatographic condition, R-ibuprofen, S-ibuprofen and indomethacin had no interference within each other. The calibration curve was linear in the range of 0.01-5 mg·L-1 for R-ibuprofen and S-ibuprofen. The relative recoveries of R-ibuprofen and S-ibuprofen were 99.1%-102.2% and 99.3%-102.4% respectively. The absolute recoveries of R-ibuprofen and S-ibuprofen were 66.8-72.9 and 66.3-71.4 respectively; RSD range of intraday were 4.2%-5.4% and 3.6%-5.1%. RSD ranges of interday were 5.9%-7.3% and 6.2%-7.9% respectively. CONCLUSION This method is accurate, rapid, with high sensitivity and good repeatability. It is applicable for the determination and pharmacokinetic study of the enantiomers of ibuprofen in plasma.

关 键 词：高效液相色谱法 布洛芬 手性固定相 对映体 

分 类 号：R917.101[医药卫生—药物分析学]