转双价基因棉花对根际土壤酶活性和养分含量的影响  

Effects of Transgenic Double Gene Cotton on the Enzyme Activity and Nutrient Content in the Rhizosphere Soil

作　　者：风春[1,2] 赵建宁 李刚 杨志国[3] 王慧 吴冬梅[4] 红雨[1] 杨殿林 

机构地区：[1]内蒙古师范大学生命科学与技术学院,呼和浩特010022 [2]农业部环境保护科研监测所,农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点开放实验室,天津300191 [3]山西农业大学生命科学院,山西太谷030801 [4]中国农业科学院棉花研究所,河南安阳455000

出　　处：《棉花学报》2013年第2期178-183,共6页Cotton Science

基　　金：转基因生物新品种培育重大专项(2011ZX08011-002);中央级公益性科研院所基本科研业务费专项资金(农业部环境保护科研监测所);农业部产地环境质量重点实验室/天津市农业环境与农产品安全重点实验室开放基金项目

摘　　要：在田间试验条件下,以3种转双价基因棉和常规棉石远321为研究对象,比较分析转双价基因棉和常规棉石远321根际土壤酶活性及养分的变化。结果表明,转双价Cry1Ac+CpTI基因棉sGK321与石远321根际土壤速效磷和铵态氮含量无显著差异,而硝态氮含量则显著高于石远321;转双价Cry1Ac+Cry2Ab基因棉(双Bt抗虫棉)速效磷和铵态氮含量均显著低于石远321,而硝态氮含量与石远321无显著差异;转双价Cry1Ac+Epsps基因棉(抗虫抗除草剂棉)速效磷和硝态氮含量均显著高于石远321,而铵态氮含量显著低于石远321。sGK321棉与石远321根际土壤脲酶、碱性磷酸酶和过氧化氢酶活性均无显著差异;双Bt抗虫棉土壤脲酶活性显著低于石远321,碱性磷酸酶和过氧化氢酶活性与石远321均无显著差异;抗虫抗除草剂棉与石远321土壤脲酶活性无显著差异,碱性磷酸酶活性显著高于石远321,而过氧化氢酶活性显著低于石远321。表明sGK321棉与石远321根际土壤养分(硝态氮除外)含量和酶活性无显著差异,而双Bt抗虫棉和抗虫抗除草剂棉所呈现的差异是因不同品种所致。Through field experiment, the enzyme activity and nutrient content in the rhizosphere soil of transgenic cotton sGK321 （CrylAc＋CPT1）, transgenic cotton with double insect-resistant genes （CrylAc＋Cry2Ab）, transgenic cotton with insect-resistant and herbicide-resistant genes （CrylAc＋Epsps） and non-transgenic cotton were analyzed. The results showed that the available phosphorus and ammonium nitrogen in the rhizosphere soil had no significant difference between transgenic cotton sGK321 and non-transgenic cotton, while the nitrate nitrogen significantly higher than non-transgenic cotton. The available phosphorus and ammonium nitrogen of transgenic cotton with double insect-resistant genes were both significantly lower than non-transgenic cotton while the nitrate nitrogen showed no difference. The available phosphorus and nitrate nitrogen of transgenic cotton with insect-resistant and herbicide-resistant genes were both significantly higher than non-transgenic cotton, but nitrate nitrogen was significantly lower than non-transgenic cotton. The urease, alkaline phosphatase and catalase activities in the rhizosphere soilshowed no significant difference between transgenic cotton sGK321 and non-transgenic cotton. Except that the urease was significantly lower than non-transgenic cotton, the alkaline phosphatase and catalase activities of the transgenic cotton with double insect-resistant genes showed no difference. For the transgenic cotton with insect-resistant and herbicide-resistant genes, the urease showed no significant difference compared with non-transgenic cotton, with the alkaline phosphatase significantly higher and the catalase activities significantly lower than non-transgenic cotton. The soil nutrient content（except nitrate nitrogen） and soil enzyme activity in the rhizosphere soil of transgenic double gene cotton sGK321 and non-transgenic cotton had no significant difference, but difference among transgenic cotton with double insect-resistant genes and transgenic cotton with insect-resi

关 键 词：转双价基因棉 根际土壤 土壤酶活性 土壤养分 

分 类 号：S562[农业科学—作物学]