海南省和河南省发生甜瓜褪绿黄化病的分子鉴定  

Molecular identification of Cucurbit chlorotic yellow virus in Hainan and Henan province

作　　者：刘珊珊[1] 彭斌[1] 吴会杰[1] 柳唐镜[2] 孔祥义[2] 施艳[3] 古勤生[1] 

机构地区：[1]河南省果树瓜类生物学重点实验室.中国农业科学院郑州果树研究所,郑州450009 [2]三亚市南繁科学技术研究院,海南三亚572000 [3]河南农业大学植物保护学院,郑州450002

出　　处：《果树学报》2013年第2期291-293,共3页Journal of Fruit Science

基　　金：现代农业产业技术体系(CARS-26-No.13)

摘　　要：【目的】为了探明引起海南省和河南省甜瓜褪绿黄化的病因,【方法】提取从海南三亚和河南郑州采集的甜瓜叶片的RNA,用CCYV特异性引物进行外壳蛋白基因的克隆、测序,并与GenBank中同属或同种病毒的cp基因核苷酸序列构建系统进化树。【结果】结果显示,RT-PCR产物经琼脂糖凝胶电泳,所得条带在880 bp左右,与预期的一致;构建cp基因的系统进化树发现,样品与瓜类褪绿黄化病毒(Cucurbit chlorotic yellows virus,CCYV)的亲缘关系很近,属于同一个分枝,而与该病毒同属不同种的南瓜黄矮失调病毒(Cucurbit yellow stunting disorder virus,CYSDV)和大麦黄矮病毒(Barley yellow dwarf virus,BYDV)的亲缘关系较远,属于不同的分枝。【结论】证实海南省和河南省甜瓜褪绿黄化病是由瓜类褪绿黄化病毒引起。[Objective] To investigate the pathogenic virus causing muskmelon yellowing in Hainan and Henan province, [Methods]the total RNA was extracted from samples collected from infected plants in Sanya, Hainan province and Zhengzhou, Henan province and the coat protein genes（cp） were amplified using Cucurbit cholorotic yellow virus （CCYV） specific primers and cloned. A phylogenetic tree was con- strueted based on the nucleotide sequences of those cp genes and all the sequences of cp genes within same species and same genus submitted in the GenBank. [Results]The results showed that the fragments of about 880 bp in size produced by RT-PCR were as expected. The phylogenetic tree showed that the samples belonged to the same branch with Cueurbit ehlorotic yellow virus whereas Cueuibit yellow stunting disorder virus（CYSDV） and Barley yellow dwarf virus（BPYV） were in other branches. [Conclusions] Our investigation suggested that the yellowing of muskmelon in Hainan and Henan province was caused by Cucurbit cholorotic yellow virus.

关 键 词：甜瓜 瓜类褪绿黄化病毒 外壳蛋白基因 黄化 

分 类 号：S652[农业科学—果树学]