转基因玉米Bt176细胞毒性及遗传毒性研究  

Study on cyto-genotoxicity of genetically modified corn Bt 176

作　　者：郑伟[1] 吕沁风[1] 吴忠华[1] 陈吴健 徐琦[1] 张明哲 吴志毅 李禾[1] 

机构地区：[1]浙江国际旅行卫生保健中心,浙江杭州310006 [2]浙江省检验检疫科学技术研究院,浙江杭州310006

出　　处：《河南农业大学学报》2013年第1期10-15,共6页Journal of Henan Agricultural University

基　　金：国家质检总局科技计划项目(2009IK162);质检公益性行业科研专项项目(201010043);浙江省食品安全重点实验室2010年科研项目(KFSZ201008)

摘　　要：利用细胞-遗传毒理学技术评价转基因玉米Bt 176细胞毒性及遗传毒性,分别以200,100,50,25,12.5mg·L-1转基因玉米Bt 176全蛋白质作用于人淋巴细胞,并各孵育1.5,6,24 h.检测其细胞毒性损伤及遗传毒性损伤,并与空白组、阳性对照组、非转基因玉米组相比较.结果表明,阳性组与空白组淋巴细胞的遗传及细胞损伤指标存在显著差异.转基因玉米组淋巴细胞遗传及细胞损伤指标与非转基因玉米组淋巴细胞相比无明显差异(P>0.05),且与空白剂组细胞差异不显著(P>0.05).结果还表明,转基因玉米Bt 176与非转基因玉米两者在遗传毒性及细胞毒性上性状相似,实质等同.To study the cyto-toxicity and genotoxicity of genetically modified（GM） corn Bt 176 by cyto-genotoxicity technology assay, human lymphocytes were exposed to whole protein of GM corn Bt 176 at doses of 200,100,50,25,12.5 mg·L^-1 and incubated for 1.5,6 and 24 h,respectively. The cyto-toxicity and genotoxicity of lymphocytes induced by GM corn Bt 176 which measured by different cyto- genotoxicity technology assay were compared with those of blank group, positive group and non GM corn group. The results showed that the cyto-genotoxicity of lymphocytes in positive control group was significantly higher than that of lymphocytes in blank control group （P 〈 0. 05 ）. There was no significant difference in cyto-genotoxicity between GM corn Bt 176 group and non-GM corn group （P 〉 0.05）. Also, the GM corn Bt 176 group did not show higher cyto-genotoxicity than that of non-GM corn group （P 〉 0.05）. This result indicates that GM corn Bt 176 has similar cyto-genotoxicity with non-GM corn.

关 键 词：Bt176 转基因玉米 细胞-遗传毒理学 

分 类 号：S513[农业科学—作物学]