烟夜蛾气味受体OR18基因克隆及组织特异性表达  

Molecular cloning and tissue-specific expression of odorant receptor OR18gene from Helicoverpa assulta(Guenée)

作　　者：张元臣[1] 范荫荫[1] 安世恒[1] 李为争[1] 乔奇[1] 郭线茹[1] 罗梅浩[1] 原国辉[1] 

机构地区：[1]河南农业大学植物保护学院,河南郑州450002

出　　处：《河南农业大学学报》2013年第1期49-54,共6页Journal of Henan Agricultural University

基　　金：河南省杰出青年科学基金项目(074100510013);河南农业大学科技创新基金项目(2007-CX-014)

摘　　要：应用RT-PCR和实时荧光定量PCR技术,从烟夜蛾雄虫触角中克隆气味受体目的基因,分析鉴定了其在烟夜蛾不同组织的特异性表达情况.序列分析结果表明,目的基因为新的烟夜蛾气味受体基因,命名为HassOR18(GenBank登录号:HM750915).该基因阅读框架全长1 197 bp,编码398个氨基酸,推测编码蛋白质的相对分子质量为46.6 kD,等电点为5.82.氨基酸序列比对表明,烟夜蛾气味受体HassOR18与其他夜蛾科昆虫OR18的氨基酸序列一致性均达80%以上.实时荧光定量PCR结果显示,HassOR18仅在雌雄虫触角中和雌虫喙内表达,而且在雄虫触角内的表达量远高于在雌虫触角和喙内的表达量,推测该基因可能参与性信息素的识别.By using RT-PCR and real-time fluorescent quantitative PCR techniques respectively, OR target gene was cloned from the male antennae of Helicoverpa assulta and the tissue-specific expression of the target gene in different tissues of H. assulta was analyzed and identified in this study. Sequence analysis revealed that the target gene from male antennae of H. assulta is a novel odorant receptor gene, named HassOR18 （GenBank access numbers： HM750915）. The full length of open reading frame in HassOR18 was 1 197 bp, encoding 399 amino acid residues with the predicted molecular weight and isoelectric point of 46.6 kD and 5.82, respectively. Amino acid sequence analysis showed that the identity of HassOR18 with OR18 from other Noctuid moths was over 80%. Real time fluorescent quantitative PCR results showed that HassOR18 transcript was observed only in the antennae of both male and female adult, and female proboscises. Moreover, the transcript expression level of HassOR18 in male antennae was much higher than that in female antennae and female proboscises. These findings suggest that HassOR18 may be involved in the recognition of sex pheromone.

关 键 词：烟夜蛾 气味受体 基因克隆 序列分析 组织特异性表达 

分 类 号：Q966[生物学—昆虫学]