HPLC法测定人血清中左乙拉西坦的浓度  

Determination of Levetiracetam Concentration in Human Serum by HPLC

作　　者：李惠芬[1] 张玉琴[1] 刘彦[2] 

机构地区：[1]天津市儿童医院,天津300074 [2]天津市人民医院,天津300121

出　　处：《中国药房》2013年第14期1272-1274,共3页China Pharmacy

基　　金：天津市卫生局科技基金资助项目(No.2011KZ35)

摘　　要：目的:建立测定人血清中左乙拉西坦浓度的方法。方法:血样经甲醇去蛋白处理后,采用高效液相色谱法进样测定。色谱柱为SunFire C18,流动相为50 mmol/L磷酸盐缓冲液(pH4.5)-乙腈(93.5∶6.5),流速为1.0ml/min,检测波长为205nm。结果:左乙拉西坦血药浓度在2.0～60.0mg/L范围内线性关系良好(r=0.9997),最低检测限为0.5mg/L;低、中、高3种质量浓度血样平均回收率为97.72%、97.67%、97.82%,日内RSD为2.74%、1.86%、1.63%,日间RSD为4.21%、1.88%、2.24%。18例患儿[给药量:左乙拉西坦片30～60mg(/kg·d)]血药浓度测得值为2.8～20.1mg/L,平均为(9.53±6.12)mg/L,个体差异大。结论:本方法精密、准确、特异性强、回收率高。OBJECTIVE： To establish a method for the determination of levetiracetam concentrations in human serum. METH- ODS： Serum samples were deproteinized with methanol. HPLC method was adopted. SunFire C18 column was used with mobile phase consisted of 50 mmol/L phosphate buffer （pH 4.5）-acetonitrile （93,5：6.5） at the flow rate of 1.0 ml/min. The detection wave- length was set at 205 nm. RESULTS： The linear range of levetiracetam was 2.0-60.0 mg/L（r=0.999 7）, and the lowest limit of de- tection was 0.5 mg/L; at three concentrations, the average recoveries were 97.72%, 97.67% and 97.82%, respectively; RSD of in- tra-day were 2.74%, 1.84% and 1.62%; RSD of inter-day were 4.21%, 1.89 and 2.24% respectively. Serum concentrations of le- vetiracetam were 2.8-20.1 mg/L in 18 children [Levetiracetam tablets 30-60 mg/（kg·d）] with average concentration of （9.53 ± 6.12） mg/L, showing significant individual difference. CONCLUSIONS： The method is precise, accurate and specific with sound recov- ery.

关 键 词：左乙拉西坦 高效液相色谱法 血药浓度 

分 类 号：R969.1[医药卫生—药理学] R971.6[医药卫生—药学]