中国朝鲜族人群MDR1基因多态性与环孢素A谷浓度的相关性研究  

Study on Correlation of Genetic Polymorphisms of MDR 1 on Trough Concentration of Cyclosporine A in Chinese Korean Patients

作　　者：唐哲[1] 孙玉坤[1] 杨媛媛[2] 何伟[1] 张敬一[3] 

机构地区：[1]武警辽宁省总队医院药剂科,沈阳110034 [2]沈阳市苏家屯区中医医院药剂科,沈阳110101 [3]沈阳军区总医院药剂科,沈阳110016

出　　处：《中国药房》2013年第14期1281-1283,共3页China Pharmacy

摘　　要：目的:研究中国朝鲜族肾移植术后患者服用环孢素A(CsA)的谷浓度与多药耐药基因(MDR1)C3435T多态性的相关性。方法:选择中国朝鲜族同种异体肾移植术后患者75例,采用聚合酶链反应(PCR)扩增MDR1C3435T基因,利用DNA限制性片段长度多态性(RFLP)方法对MDR1C3435T基因进行分型,结合口服CsA的谷浓度,判断二者是否存在关联。结果:75例患者中,C/C型31例(41.3%),C/T型37例(49.3%)和T/T型7例(9.3%)。C3435T基因多态性与CsA谷浓度差异无统计学意义(P>0.05)。结论:C3435T基因多态性可能不是影响中国朝鲜族肾移植术后患者CsA谷浓度的主要因素。OBJECTIVE： To study the correlation between the polymorphisms of multidrug resistance gene （MDR1） C3435T and trough concentration of cyclosporine A（CsA）in Chinese Korean patients after renal transplantation. METHODS： 75 Chinese Ko- rean patients underwent renal allografts were investigated. The amplification and genotyping of MDR1 C3435T geno-type was con- ducted by PCR and RFLP assays. Based on trough concentration of CsA with oral administration, possible associations of gene poly- morphisms and trough concentration of CsA were confirmed. RESULTS： Among 75 patients, 31 （41.3%）, 37 （49.3%） and 7 （9.3%） cases were carriers of C/C, C/T and T/T. There was no statistical difference between C3435T polymorphisms and trough concentrations of CsA （P〉0.05）. CONCLUSIONS： C3435T gene polyrnorphism might not be the main influential factor of CsA trough concentrations in Chinese Korean patients.

关 键 词：环孢素A 多药耐药基因 基因多态性 中国朝鲜族 

分 类 号：R968[医药卫生—药理学] R979.5[医药卫生—药学]