CdTe量子点荧光猝灭法测定槲皮素  

Determination of quercetin based on fluorescence quenching of CdTe quantum dots

作　　者：陈志兵[1] 李梦瑶[1] 孙莉[2] 胡莉萍[1] 

机构地区：[1]宿州学院化学与生命科学学院,宿州234000 [2]安徽省芜湖市食品药品检验所,芜湖241001

出　　处：《分析试验室》2013年第4期17-19,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金项目(21271136);安徽省教育厅重点项目(KJ2012A267);宿州学院科研平台开放课题(2012YKF01)资助

摘　　要：以巯基乙酸修饰的CdTe量子点为荧光探针,考察了不同条件下槲皮素对CdTe量子点的猝灭作用。发现在磷酸缓冲溶液中,其猝灭作用最强。在此基础上建立了基于荧光猝灭法检测槲皮素含量的新方法,该方法的线性范围为0～3.5×10-5 mol/L,线性方程为△F=42.86+36.98 c(×10-6mol/L),相关系数r为0.9985,检测限(3SD/斜率)为1.7×10-7mol/L。方法应用于降脂宁颗粒和洋葱中槲皮素含量的测定,相对标准偏差为2.1%和2.3%,回收率在98%～102%之间。The fluorescence quenching of quercetin to thioglycolic acid stabilized CdTe quantum dots（QDs） has been systematically investigated by using CdTe QDs as a fluorescence probe. On the basis of that, a sensitive method for the determination of quercetin was developed. The linear equation was △F =42.86 ＋36.98 c （ ×10^-6mol/L） , and its linear range was 0 - 3.5 ×10^-5 mol/L with a correlation coefficient of 0. 9985. The limit of detection was 1.7 ×10^-7 mol/L. The method was applied to the determination of quercetin in Iiangzhining grains and onion. The relative standard deviations of results were 2. 1% and 2. 3 %, and the values of recovery test obtained by the standard addition method were in the range of 98% - 102%.

关 键 词：量子点 槲皮素 荧光猝灭 

分 类 号：O657.3[理学—分析化学]