MircoRNA156家族在小麦非生物胁迫中的表达分析  

Expression analysis of wheat mircoRNA156 family in response to abiotic stress

作　　者：王冰[1] 宋娜[1] 孙燕飞[1] 冯浩[1] 王晓杰[1] 康振生[1] 

机构地区：[1]西北农林科技大学植物保护学院旱区作物逆境生物学国家重点实验室,杨凌712100

出　　处：《植物病理学报》2013年第2期201-204,共4页Acta Phytopathologica Sinica

基　　金：国家自然科学基金(31071651);现代农业产业技术体系建设专项(nycytx-03);高等学校学科创新引智计划(B07049)

摘　　要：MircoRNA（miRNA）作为一类20碱基左右的非编码RNA，它在转录水平调控基因的表达，在植物抗逆境胁迫的调节网络中发挥了重要作用。通过对小麦miRNAs的研究，可以扩展小麦抵抗自然灾害的途径，减少损失。有报道表明miRl56作为保守的miRNA在多种植物中参与到抗逆反应，The objective of this study was to identify the roles of miR156 family in different abiotic surround- ings in wheat （ Triticum aestivum L）, reveal the cross-talk of members of miR156 family in abiotic regulatory networks and better understand the mechanisms responsible for abiotic stress tolerance. Five members of miR156 family were found in wheat through Solexa high-throughput sequencing. The Northern blot results showed that the expressions of miR156a and miR156d ＊ were up-regulated in UV-B stress, but the expression pattern of miR156c ＊ and miR156b ＊ were significantly down-induced after 24 hours with cold and UV-B treat- ment. Furthermore, the qRT-PCR analyses showed the expressions of miR156 family were different after trea- ted by every abiotic stress. These results indicated that some members of miR156 family may play different roles in response to abiotic stress.

关 键 词：非生物胁迫 表达分析 小麦 MIRNAS 家族 非编码RNA 调控基因 转录水平 

分 类 号：S511.03[农业科学—作物学]