烟草靶斑病菌菌丝融合群及ITS序列分析  

The anastomosis groups and ITS sequence analysis of Rhizoctonia solani isolates of tobacco target spot

作　　者：吴元华[1] 伏颖[1] 赵秀香[1] 穆凌霄[1] 赵艳琴[1] 

机构地区：[1]沈阳农业大学植物保护学院,沈阳110866

出　　处：《植物病理学报》2013年第2期215-218,共4页Acta Phytopathologica Sinica

基　　金：国家烟草专卖局科技项目"全国烟草有害生物调查研究"(国烟办综(2010)182号);辽宁省烟草专卖局科技攻关项目"辽宁省烟草有害生物调查研究"(辽烟计(2010)86号)

摘　　要：烟草靶斑病是2006年我国新报道发生的一种叶部病害，其病原的无性世代为立枯丝核菌（Rhizoctonia solaniKfihn），有性世代为瓜亡革菌（Thanatephorus cucumeris（Frank）Donk）。该病菌主要危害叶部形成病斑，对烟草的产量和品质影响显著，目前该病害主要分布在辽宁省丹东和铁岭地区，并呈现出迅速蔓延趋势。烟草靶斑病最早由巴西报道，此后，哥斯达黎加、美国、南非和津巴布韦也相继发生。Fifty-eight isolates of Rhizoctonia solani were isolated from tobacco target spot disease samples col- lected from tobacco growing areas in Liaoning Province. The test results of anastomosis groups （AGs） by glass slide fix position mating method showed that all isolates of R. solani belonged to AG-3. The rDNA ITS se- quence of the R. solani strain YC-9 was amplified and sequenced by using the universal primers ITSland ITS4, and compared by nucleotide BLAST with other nucleotides in GenBank. It was showed that the ITS sequence of yC-9 （653 bp） was completely identical to that of the R. solani AG-3 isolate from tomato foliar blight. The re- sults provide important basis for further investigation of tobacco target spot disease.

关 键 词：靶斑病菌 ITS序列分析 菌丝融合群 烟草 叶部病害 立枯丝核菌 哥斯达黎加 无性世代 

分 类 号：S436.421.1[农业科学—农业昆虫与害虫防治]