芫菁体内斑蝥素和结合斑蝥素抗肿瘤活性的比较研究  

Comparative study on anti-tumor effect of cantharidin and bound cantharidin from Meloidae

作　　者：李晓飞[1] 娄方明[2] 晏容[1] 侯晓晖[1] 张恒[1] 刘云[3] 

机构地区：[1]遵义医学院基础医学院,贵州遵义563003 [2]遵义医学院药学院,贵州遵义563003 [3]遵义医学院医学与生物学研究中心,贵州遵义563003

出　　处：《时珍国医国药》2013年第3期535-538,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81260488);贵州省优秀科技教育人才省长资金(黔省专合字[2010]48号);贵州省中药现代化科技产业研究开发专项项目(黔科合社字[2009]5042);贵州省科技厅社会发展攻关项目(黔科合SY字[2011]3031)

摘　　要：目的比较提取自眼斑芫菁体内的斑蝥素和结合斑蝥素的体外抗肿瘤活性。方法将提取自眼斑芫菁体内的斑蝥素和结合斑蝥素作用于人结肠癌细胞HT-29、人肝癌细胞PLC/PRF/5、人肝癌细胞BEL-7404;采用WST-1比色法进行体外细胞抑制实验,测定斑蝥素和结合斑蝥素对上述三种肿瘤细胞的生长抑制率。结果斑蝥素对HT-29结肠癌细胞的抑制作用不稳定,对PLC/PRF/5和BEL-7404肝癌细胞的抑制作用随用药浓度的增加而增强,但在高浓度作用下仍表现出较低抑制率。结合斑蝥素对HT-29、PLC/PRF/5、BEL-7404三株肿瘤细胞均表现出明显的抑制效果,且随药物浓度升高其抑制作用增强,呈剂量效应关系;其半数抑制浓度分别为:28.51,86.77,21.27μmol/L。结论芫菁体内结合斑蝥素对人结肠癌细胞HT-29、人肝癌细胞PLC/PRF/5、人肝癌细胞BEL-7404的抑制作用远好于斑蝥素,提示结合斑蝥素的抗癌效果极可能好于斑蝥素。Objective To compare the anti-tumor activity of cantharadin and the bound cantharidin extacted from meloids Mylabris cichorii on various human tumor cell lines in vitro.Methods WST-1 colorimetry was used to evaluate the inhibitory effect of cantharadin and the bound cantharidin on HT-29 cells,PLC/PRF/5 cells and BEL-7404 cells.Results Cantharidin displayed unstable inhibitory effect on the growth of HT-29 cells,while their inhibitory effect on the growth of PLC/PRF/5 cells and BEL-7404 cells was in a dose-dependent manner,but with a little activity under the high concentration.The bound cantharidin was able to effectively inhibit the proliferation of three kinds of the tumor cells with the IC50 value of 28.51 μmol/L,86.77 μmol/L and 21.27 μmol/L,respectively,in a dose-dependent manner.Conclusion The bound cantharidin showed more effective inhibitory effect on HT-29 cells,PLC/PRF/5 cells and BEL-7404 cells compared with cantharidin.The results of assay suggested that roles of the bound cantharidin could be more significantly contributed to anti-tumor than the roles of cantharidin.

关 键 词：斑蝥素 结合斑蝥素 抗肿瘤 半数抑制浓度 

分 类 号：R284.2[医药卫生—中药学]