糖尿病周围神经病变小鼠神经生长因子及其受体的变化及周络通胶囊的干预作用  

The change of nerve growth factor and its receptor of Diabetic peripheral neuropathy mice and the intervention effect of ZhouLuotong capsule

作　　者：王宏涛 张会欣 赵韶华[2] 魏刚 何奇龙[1] 高学东[2] 朱慧明 

机构地区：[1]河北以岭医药研究院,河北省络病重点实验室,国家中医药管理局重点研究室,河北石家庄050035 [2]石家庄以岭药业股份有限公司,河北石家庄050035

出　　处：《时珍国医国药》2013年第3期547-549,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家十二五“重大新药创制”科技重大专项(No.2011ZX09101-004-02);河北省中医药管理局科研计划项目(No.2011041)

摘　　要：目的观察糖尿病周围神经病变(DPN)小鼠神经生长因子(NGF)及其受体的变化及周络通胶囊的干预作用。方法 40只KK/Upj-Ay小鼠随机分为模型组、周络通胶囊高、中、低剂量组(6.85,3.43,1.71 g生药.kg-1),另设10只C57BL/6小鼠对照组。灌胃给药12周,测定运动神经传导速度(MNCV);荧光定量PCR和Western blot法测定坐骨神经NGF、高亲和力受体Trk A、低亲和力受体p75NTR表达。结果模型组与对照组比较,MNCV明显下降(P<0.01),NGF、Trk A、p75NTR表达明显下降(P<0.01);与模型组比较,周络通组能提高MNCV(P<0.05,P<0.01);升高NGF、Trk A、p75NTR表达(P<0.05,P<0.01)。结论周络通胶囊对DPN小鼠有保护作用,与升高NGF及其受体TrkA、p75NTR表达有关。Objective To investigate the change of nerve growth factor and its receptor of diabetic peripheral neuropathy mice and the intervention effect of ZhouLuotong capsule（ZLT）.Methods 40 KK/Upj-Ay mice were randomly divided into vechicle group,different dose ZLT group（low,middle,high）.C57BL/6 mice were selected as control group.Mice were given intragastrically for 3 months.MNCV were detected by BL-420E.Real Time PCR and Western blot were used to assess NGF,high affinity receptor Trk A,low affinity p75NTR receptor mRNA and protein expression in sciatic nerves of DPN rats.Results Compared with controls,MNCV of vechicle group declined obviously（P 0.01）;NGF,Trk A,p75NTR expression declined obviously（P0.01）.Compared with vechicle group,the deferent dose ZLT group can improve MNCV（P0.05,P0.01） and elevate NGF,Trk A,p75NTR expression（P0.05,P0.01）.Conclusion The protect effect of ZLT on DPN mice may be relevant to elevating NGF and its receptor TrkA,p75NTR expression.

关 键 词：周络通胶囊 糖尿病周围神经病变 神经生长因子 神经生长因子受体 

分 类 号：R587.2[医药卫生—内分泌]