α_(1)(I)型前胶原基因反义核酸对增生瘢痕动物模型的抑制作用  

Inhibiting effect of antisense oligodeoxynucleotide of a_(1)(I)precollagen gene on hypertrophic scar in an animal model

作　　者：祁少海[1] 利天增[1] 单越新[2] 罗超权[2] 黎志明[1] 谢举临[1] 吴义方[3] 

机构地区：[1]中山医科大学附属第一医院烧伤科,510080 [2]中山医科大学生物化学教研室,510080 [3]中山医科大学生物化学电镜室,510080

出　　处：《中华整形外科杂志》2000年第5期295-297,共3页Chinese Journal of Plastic Surgery

基　　金：广东省重点科技攻关项目资助

摘　　要：研究ａ１（Ｉ）型前胶原基因反义寡聚核苷酸对人烧伤所致增生性瘢痕裸鼠模型的作用，探讨增生性瘢痕的基因治疗。方法以人烧伤增生性瘢痕移植于裸鼠建立动物模型，分别合成寡聚核苷酸１（ＯＤＮ１）和寡聚核苷酸２（ＯＤＮ２），作用于动物模型，观察不同寡聚核苷酸的抑制作用。结果位于５’端翻译区域的２１ｂｐ的反义寡聚核苷酸１和位于第１个外显子与第１个内含子之间的２２ｂｐ的反义寡聚核苷酸２能有效抑制增生性瘢痕的生长，对照的ＲＭＰＩ组抑制作用不明显，空白对照组无变化。结论作用于５’端翻译区域的反义寡聚核苷酸和作用于第１个外显子与第１个内含子之间的反义寡聚核苷酸能有效抑制Ｉ型胶原蛋白的合成，从而抑制瘢痕增生。Objective To study the effect of antisense oligodeoxynucleotide (ODN) of a1 (I) precollagen gene on hypertrophic scar in an animal model. Method Hypertrophic scar was explanted in athymic mice. The effect of antisense oligodeoxynucleotide 1 and 2 on the animal model and the inhibition of ODN1 and ODN2 were observed. Result it was showed that antisense oligo (ODN1, 21bp) located 5'end from the translation start region and antisense oligo (ODN2, 22bp) between the first exon and the first intron could effectively inhibit scar hypertrophy. However the control groups showed no inhibition. Conclusion Antisense oligodeoxynucleotide could effectively inhibit scar hypertrophy by inhibiting the synthesis of type I collagen protein.

关 键 词：I型胶原基因 反义寡聚核苷酸 瘢痕 

分 类 号：R619[医药卫生—外科学]