同种异体组织工程化软骨组织形成的初步研究  

An initial experiment and study on allogenic tissue engineered cartilage

作　　者：夏万尧 曹谊林[1,2,3] 商庆新[1,2,3] 刘彦春 王倬[1,2,3] 崔磊 王健[1,2,3] 

机构地区：[1]上海市第二医科大学组织工程研究中心,200011 [2]上海市组织工程研究重点实验室,200011 [3]上海第二医科大学附属第九人民医院整形外科,200011

出　　处：《中华整形外科杂志》2000年第5期264-266,共3页Chinese Journal of Plastic Surgery

基　　金：上海市科学技术委员会基金!(98Jc14026)

摘　　要：探讨同种异体组织工程化软骨在动物体内形成。方法　取怀孕１００ｄ胚胎羊的关节软骨经体外消化分离软骨细胞，与可吸收生物材料ＰｌｕｒｏｎｉｃＦ１２７混合形成细胞──生物材料复合物，将细胞──生物材料复合物注射到异体羊腹壁皮下，每隔２周取材，连续半年对所取标本进行大体观察、称重，并作 ＨＥ、Ｓａｆｒａｎｉｎ Ｏ、Ｍａｓｓｏｎ’ｓ ｔｒｉｃｈｒｏｍｅ染色，对再生的软骨组织进行评价。结果 在同种异体动物体内能形成软骨组织，所形成软骨组织的氨基多糖和胶原分布与正常羊关节软骨组织有相似的组织学特性。组织学结果可见第２，４周的软骨组织有一定程度的免疫排斥反应和炎症细胞浸润。第６周后软骨组织基本成熟，免疫反应减轻，但未消退。结论　应用组织工程技术可在同种异体动物体内再生软骨组织，再生的组织周围存在一定程度的免疫反应，随接种时间的延长逐渐减弱。Objective To investigate the formation of engineered cartilage in vivo with allogenic chondrocytes. Methods and Materials Joint cartilage from ram embryos (Pregnancy: 100 days) was isolated and digested to obtain dissociated chondrocytes. Mixed chondrocytes with biomaterials (Pluronic 127) at the density 50×106/ml, then implant the allograft complex subcantenously. Collect the novel tissue every two weeks along for six months, weigh and stain (HE.Safranin O. Masson's trichrome) the sample tissues individually to evaluate the characteristics of novel tissue. Result Novel cartilage can regenerate in allograft animals, with similar histological properties of chondrocytes, aminoglycin and collagen distribution to normal cartilage. The novel cartilage observed histologically showed apparent surrounding inflammatory cells in two and four week and the tissue of cartilage came to mature since the sixth week, with less inflammatory reaction, but not disappeared. Conclusion Novel cartilage can generate in allograft animals with tissue engineering approach, with certain immunoreaction surrounding. Such reaction will be weakened gradually with time.

关 键 词：软骨细胞 软骨组织 同种异体 组织工程化复合物 

分 类 号：R318.0[医药卫生—生物医学工程]