成年大鼠联体诱导的移植耐受与嵌合状态相关性的研究  

Correlation of transplantation tolerance and chimerism induced by allogeneic parabiosis in adult rats

作　　者：史其新[1] 谢蜀生[2] 俞莉章[1] 郭应禄[1] 

机构地区：[1]北京医科大学第一医院泌尿外科北京医科大学泌尿外科研究所,100034 [2]北京医科大学免疫学系

出　　处：《中华医学杂志》2000年第8期602-605,共4页National Medical Journal of China

摘　　要：目的　将异基因成年大鼠联体共生 (parabiosis)以诱导相互特异的移植耐受 ,探讨嵌合体与耐受的关系。方法　联体前后低剂量环磷酰胺 (CP ,l0 0mg/kg)腹腔注射 ,观察联体大鼠存活时间。分别于联体 5、15、45d时将联体大鼠分开 ,并相互植皮 ,观察皮肤存活情况 ,并在上述时间点以流式细胞仪检测联体分开后大鼠胸腺、脾脏中供体来源细胞的嵌合状态。在联体 15d时检测同种异型混合淋巴细胞反应 (MLR)、迟发性超敏反应 (DTH)。结果　实验组大鼠联体存活时间 (中位数 =6 3.5d ,P =0 .0 0 0 5 )明显延长。DA、LEW大鼠于联体 15d可诱导较稳定的皮肤移植耐受。嵌合状态在移植耐受诱导期 (15d内 )呈明显增长 ,而在耐受维持期 (15d后 )嵌合状态无明显变化。联体 15dMLR、DTH呈供体特异性降低。结论　联体共生可诱导DA、LEW大鼠相互特异的移植耐受 ,嵌合体与耐受状态有明显的相关性。Objective To induce transplantation tolerance by allogeneic parabiosis in adult rats to explore the relation between chimeras and tolerance. Methods Cyclophosphamide(CP, 100 mg/kg) was intraperitonially administered before and after parabiosis to observe the survival days of parabiotic rats. Grafting skin for each other after separation of parabiosed rats pairs on day 5, 15 and 45 respectively to observe the survival time of skin graft survival. In the same time the percentage of cells of donor origin in the spleen and thymus of recipient rats were determined by FACS. Mixed lymphocyte reaction(MLR) and delayed type hypersensivity(DTH) at day 15 of parabiosis were tested. Results Surival days of parabiotic rats were significantly prolonged ( P =0.000 5, MST=63.5 days). After 15 day of parabiosis, the stable skin transplantation tolerance was established. During the first 15 day of parabiosis, the level of chimerism was increased with time, afterwards the chimerism became relatively stable. DTH and MLR of parabiotic rats on day 15 were donor specifically suppressed. Conclusion Parabiosis can induce specific trallsplantation tolerance in both parabiotic DA and LEW rats. There is a positive correlation of chimerism and transplantation tolerance.

关 键 词：嵌合体 移植耐受 嵌合状态 移植免疫学 

分 类 号：R392.1[医药卫生—免疫学]