藏药蔓菁抗氧化活性多糖的提取及纯化工艺优选  

Optimization of Extraction and Purification Technology of Antioxidant Activity Polysaccharides from Brassica rapa by Orthogonal Design

作　　者：杨永东[1] 李聪颖[1] 唐策[1] 王张[1] 范刚[1] 张艺[1] 

机构地区：[1]成都中医药大学民族医药学院,成都611137

出　　处：《中国实验方剂学杂志》2013年第7期7-10,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30960507);四川省中医药管理局科研项目(2012-G-029);四川省教育厅四川省省属高校科研创新团队建设计划项目(11TD004);成都中医药大学科技发展基金项目(CGZH201201)

摘　　要：目的:优化藏药蔓菁中抗氧化活性多糖的提取、纯化工艺。方法:以粗多糖得率、总多糖质量分数和抗氧化活性的综合评分为指标,采用正交试验考察浸提次数、料液比及浸提时间对蔓菁多糖提取工艺的影响;以色素去除率和多糖损失率为综合评价指标,通过正交试验考察脱色温度、吸附时间、活性炭用量对脱色工艺的影响。结果:蔓菁多糖最佳提取工艺为料液比1∶30,于90℃水浴浸提3次,每次2 h;最佳脱色工艺为加3%活性炭于60℃吸附40 min。蔓菁多糖纯度达4.66%,其清除DPPH自由基的IC50=6.71 g.L-1。结论:优选的提取、纯化工艺稳定可靠,所得多糖的含量高、杂质少,适合于蔓菁多糖的工业化生产。Objective： To optimize extraction and purification procedures of antioxidant activity polysaccharides from Brassica rapa. Method： With the crude polysaccharides yield, the content and antioxidant activity of total polysaccharides as indicators, orthogonal design was used to optimize extraction technology by taking extraction times, solid-liquid ratio and extraction time as factors ; With the removal rate of pigment and the loss rate of polysaccharides as comprehensive evaluation index, effects of bleaching temperature, adsorption time and activated carbon dosage on decoloration technology were investigated by orthogonal test. Result： Optimum extraction technology was as following ： solid-liquid ratio 1：30, extracted 3 times at 90 ~C with 2 h each time ; The best decoloration technology was： absorbed 40 rain with 3% activated carbon at 60 ~C. Under these conditions, purity of polysaccharides from B. rapa was up to 4.66% , the ICso value of DPPH free radical scavenging was 6.71 g .L-I. Conclusion： These optimized extraction and purification processes were stable and reliable, a high level of polysaccharides with fewer impurities was obtained, which indicated that optimized processes were suitable for industrial production of polysaccharides from B. rapa. from B. rapa had strong antioxidant activity, and worthy

关 键 词：藏药 蔓菁 多糖 提取工艺 抗氧化 

分 类 号：R283.6[医药卫生—中药学]