HP-20型大孔树脂富集纯化南山茶花总黄酮的工艺优选  

Optimization of Enrichment and Purification Technology for Total Flavonoids from Flowers of Camellia semiserrata by HP-20 Macroporous Resin

作　　者：尚志春[1] 宋明明[1] 史丽颖[1] 于大永[1] 唐玲[1] 王永奇[1] 

机构地区：[1]大连大学药物研究所,辽宁大连116622

出　　处：《中国实验方剂学杂志》2013年第7期11-13,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30772714)

摘　　要：目的:优选HP-20型大孔树脂富集纯化南山茶花总黄酮的工艺参数。方法:以总黄酮含量和洗脱率为指标,采用单因素试验考察HP-20型大孔吸附树脂的静态饱和吸附量、动态饱和吸附量、乙醇体积分数、乙醇用量等工艺参数。结果:HP-20型大孔树脂对南山茶花总黄酮的静态饱和吸附量50.5 mg.g-1。最佳纯化工艺为上样液体积9 BV,分别用3 BV去离子水及15%乙醇洗除杂质,35%乙醇10.5 BV以2 BV.h-1流速洗脱。总黄酮纯度达54.24%,总黄酮洗脱率29.83%。结论:HP-20型大孔吸附树脂对南山茶花总黄酮具有良好的纯化效果,优选的工艺合理、稳定可行。Objective： To optimize purification technology parameters of total flavonoids from flowers of Camellia semiserrata with HP-20 macroporous resin. Method： With elution ratio and purity of total flavonoids as indexes, technology parameters of HP-20 macroporous resin were investigated by single factor test, including static saturated adsorption amount, dynamic saturated adsorption amount, the concentration and amount of alcohol, et al. Result： Static saturated adsorption amount was 50.5 mg per gram resin. Optimum purification technology was as following： the volume of sample liquid 9 BV, washed impurity with 3 BV of deionized water and 3 BV of 15% ethanol, eluted with 10. 5 BV of 35% ethanol at flow rate of 2 BV .h-1. Under these process conditions, purity of total flavonoids was up to 54.24%, elution rate of total flavonoids was 29.83%. Conclusion： HP-20 macroporous resin had good purification effects on total flavonoids from flowers of C. semiserrata, this optimized purification process was reasonable, stable and feasible.

关 键 词：南山茶花 HP-20型大孔吸附树脂 总黄酮 工艺条件 

分 类 号：R283.6[医药卫生—中药学]