正交试验优选罗布麻叶中总鞣质提取工艺  

Optimization of Extraction Technology for Total Tannin from Leaves Apocynum venetum by Orthogonal Test

作　　者：樊珍珍[1] 热娜.卡斯木[1] 王晓梅[1] 胡君萍[1] 王新玲[1] 王小青[1] 

机构地区：[1]新疆医科大学药学院,乌鲁木齐830011

出　　处：《中国实验方剂学杂志》2013年第7期13-16,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：新疆维吾尔自治区科技支疆项目(201191259);2012年国家科技支撑计划课题(2012BAI30B02)

摘　　要：目的:优选罗布麻叶中总鞣质的提取工艺。方法:以鞣质含量为指标,采用单因素试验考察提取方式、提取溶剂、料液比等因素对提取工艺的影响;以浸膏得量和总鞣质提取量为综合评价指标,通过正交试验考察提取次数、提取时间及料液比对罗布麻叶中总鞣质提取工艺的影响,确定最佳提取工艺。结果:最佳提取工艺为A2B3C3,即加20倍量甲醇回流提取3次,每次3 h。结论:优选的提取工艺稳定可行,可作为罗布麻叶的工业化生产工艺。Objective ： To optimize extraction process of total tannins from leaves of Apocynumm venetum. Method： With the content of total tannins as index, single factor test was used to investigate extraction methods, extraction solvent, solid-liquid ratio and other factors on extraction technology ; With extract amount and yield of total tannins as comprehensive evaluation index, orthogonal test was used to optimize extraction technology of total tannins from leaves of A. venetum with liquid-solid ratio, extraction time and times as factors. Result： Optimum extraction process was A2B3C3, that was as following： reflux extracted 3 times with 20 times the amount of methanol, 3 h each time. Conclusion： This optimized extraction technology was stable, reasonable and feasible, it could be as an industrial production technology for leaves of A. venetum.

关 键 词：罗布麻叶 总鞣质 提取工艺 正交试验 

分 类 号：R283.6[医药卫生—中药学]