星点设计-效应面法优化老鹳草总黄酮的提取工艺  

Optimization of Extraction Technology for Total Flavonoids in Geranium wilfordii by Central Composite Design-Response Surface Methodology

作　　者：朱明慧[1] 尹海波[1] 王洪成[1] 陈静[1] 姚佳[1] 姜忠韬[1] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600

出　　处：《中国实验方剂学杂志》2013年第7期31-34,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：辽宁省教育厅项目(2009A498)

摘　　要：目的:采用星点设计-效应面法优化老鹳草中总黄酮的醇提取工艺。方法:以乙醇体积分数、回流时间、溶剂用量和提取温度为自变量,总黄酮得率为因变量,对自变量各水平进行多元线性回归和二项式拟合,通过效应面法优选提取工艺条件,并进行预测分析。结果:最佳提取工艺为加35倍量65%乙醇于90℃提取2次,每次70 min;总黄酮提取率的实测值与预测值偏差-1.25%,二项式拟合复相关系数0.960 9。结论:优选的醇提工艺稳定简便,且预测性良好。central composite Objective ： To design/response optimize extraction technology of total flavonoids in Geranium wilfordii, by surface methodology. Method ： Independent variables were ethanol concentration, reflux time, the amount of solvent and reflux temperature, dependent variable was extraction rate of total flavonoids, linear or no-liner mathematic models were used to estimate the relationship between independent variables and dependent variable, response surface methodology was used to optimize extraction technology, and prediction was carried out through comparing the observed and predicted value. Result： Regression coefficients of binomial fitting complex model was as high as 0. 960 9, optimal extraction technology conditions were as followings： extracted 2 times with 35 times the amount of 65% ethanol at 90℃ , 70 min per time. Bias between the observed and predicted value was- 1.25%. Conclusion： Central composite design/response surface methodology was convenient and highly predictive for optimizing extraction technology of total flavonoids.

关 键 词：老鹳草 星点设计 效应面优化法 总黄酮 提取工艺 

分 类 号：R283.6[医药卫生—中药学]