正交试验法优选骨愈搽剂的提取工艺  

Optimization of Extraction Technology for Guyu Liniment by Orthogonal Design

作　　者：温然[1] 高瑛 陆洋[1] 杜守颖[1] 赵雪姣[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京市顺义区中医医院,北京101300

出　　处：《中国实验方剂学杂志》2013年第7期39-41,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市中医药科技项目(JJ2011-36)

摘　　要：目的:优选骨愈搽剂的提取工艺。方法:以羟基红花黄色素A含量为指标,采用HPLC测定,通过L9(34)正交试验法考察乙醇体积分数、提取次数、溶剂用量、提取时间对骨愈搽剂提取工艺的影响。结果:最佳提取工艺为加12倍量70%乙醇回流提取2次,每次1.5 h。结论:优选的提取工艺稳定可行,且羟基红花黄色素A含量较高。Objective： To optimize extraction process of Guyu liniment. Method： Orthogonal test was employed to optimize extraction procedure, including the concentration of ethanol, the amount of solvent, extraction time and extracting times. The content of hydroxy safflor yellow A （HSYA） was used as index which was determined by HPLC. Result： Optimum extracting process for Guyu liniment was as follows： reflux extracted 2 times with 12 times the amount of 70% ethanol, 1.5 h per time. Conclusion： This optimized process was stable and feasible with relatively high content of HSYA.

关 键 词：骨愈搽剂 提取工艺 正交试验 羟基红花黄色素A 高效液相色谱 

分 类 号：R283.6[医药卫生—中药学]