三七中总皂苷含量测定的对照品筛选  

Screening of Reference Substances for Content Determination of Total Saponins in Panpax notoginseng

作　　者：陈旭[1,2] 党晓芳[1] 曹飒丽[1] 林龙飞[1] 倪健[1] 冯丽君[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]北京市药品监督管理局药品审评中心,北京100061

出　　处：《中国实验方剂学杂志》2013年第7期66-68,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京中医药大学复方中药制药创新团队项目(2011-CX70-13)

摘　　要：目的:考察选用不同皂苷类对照品为指标对样品中三七总皂苷含量测定的影响。方法:以人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1、三七总皂苷为对照品,香草醛-高氯酸显色,采用紫外分光光度法测定三七中三七总皂苷的含量。结果:以不同对照品为指标测定三七总皂苷含量时,结果存在明显差异。结论:紫外分光光度法测定三七及其制剂中总皂苷含量时,可用三七总皂苷作对照品,为含有三七的制剂含量测定提供较为可靠的实验数据。Objective： To investigate the effects of different control samples on content determination of total saponins from Panax notoginseng. Method： With ginsenosides Rgl, ginsenoside Rbl, notoginsenoside R1 and P. notoginseng saponins as reference substance, taking HC104-vanilin staining method, the content of total saponins in P. notoginseng was determined by ultraviolet spectrophotometry. Result： There were significant differences in determination of the content of total content from P. notoginseng among groups of different references. Conclusion： With UV spectrophotometry as content determination method for total saponins from P. notoginseng, we could choose P. notoginseng saponins as a reference substance, which could provide more reliable experimental data for determining the content of P. notoginseng and its preparations.

关 键 词：三七 人参皂苷RG1 人参皂苷RB1 三七皂苷R1 三七总皂苷 分光光度法 含量测定 

分 类 号：R283.6[医药卫生—中药学]