不同生长年限苦参不同部位的生物碱含量  

Determination of Five Alkaloids from Sophora flavescens of Different Parts and Different Growth Years

作　　者：陈静[1] 王淑美[1] 孟江[1] 孙飞[1] 王佰灵[1] 刘贝[1] 梁生旺[1] 

机构地区：[1]广东药学院中药学院,广州510006

出　　处：《中国实验方剂学杂志》2013年第7期80-84,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家中医药管理局中医药行业科研专项(201007012-1-9)

摘　　要：目的:对不同年限苦参药材茎、叶、芦头、侧根、根5个部分总生物碱与5种单体生物碱进行含量测定。方法:采用高效液相色谱法分析不同年限苦参药材不同部位中5种指标性生物碱的含量。结果:5种指标性生物碱在不同生长年限苦参不同部位的分布有不同特点,不同部位槐果碱的含量分布叶>茎>侧根>主根>芦头,苦参碱的分布是叶>茎>芦头>侧根>主根,氧化槐果碱的分布是侧根>主根>芦头>茎>叶,槐定碱的分布是侧根>主根>叶>茎>芦头,氧化苦参碱的分布是侧根>主根>芦头>茎>叶。随着生长年限的增加,各指标性生物碱成分含量随之增加,第4年有效成分增加幅度较小,其中主根中5种指标性成分含量依次为一年生13.58 mg.g-1,两年生20.49 mg.g-1,三年生27.74 mg.g-1,四年生31.32 mg.g-1。结论:上述结果为合理开发利用苦参以及苦参的生长年限提供了实验依据。Objective： To determine the five alkaloids of different parts in S. flavescens of different growth years. Method： The five alkaloids in S. flavescens were analyzed by HPLC. Result： Five alkaloids of different parts in S. flavescens of different growth years had their distribution law. The content of sophocarpine＇s distribution in different parts was leaf 〉 stem 〉 lateral root 〉 taproot 〉 reed head ; the distribution of matrine was leaf 〉 stem 〉 reed head 〉 lateral root 〉 taproot ; the distribution of oxysophocarpine was lateral root 〉 taproot 〉 reed head 〉 stem 〉 leaf; the distribution of sophoridine was lateral root 〉 taproot 〉 leaf 〉 stem 〉 reed head ; the distribution of oxymatrine was lateral root 〉 taproot 〉 reed head 〉 stem 〉 leaf. With the increase of growth years, the five alkaloids increased; the fourth year was tend to be gentle. For example, the content of the taproot was： 13.58 mg·g-1 （first year）, 20.49 mg .g-1 （second year）, 27.74 mg .g-1 （third year）, 31.32 mg .g-1 （forth year）. Conclusion ： This research can provide data for rational utilization and growth years determination of S. flavescens.

关 键 词：苦参 不同年限 不同部位 高效液相色谱法 5种生物碱 

分 类 号：R284.1[医药卫生—中药学]